Vitis thunbergii var. Taiwaniana leaf extract reduces blood glucose levels in mice with streptozotocin-induced diabetes

Wan Leung, Feng Ming Ho, Wen Pin Li, Yu Chih Liang

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

Background and Objective: Vitis thunbergii var. taiwaniana (VTT) is a native folk medicinal plant in Taiwan used to treat hepatitis, jaundice, stomachaches, diarrhea and arthritis. This study aimed to investigate the antidiabetic activity of VTT extracts using a mice model of streptozotocin (STZ)-induced diabetes. Materials and Methods: The ICR male mice were received a single i.p., injection of 100 mg kg-1 of STZ to induce type 2-like diabetes. Diabetic mice were orally administered with 100 mg kg-1 of VTT-leaf alcohol extracts, VTT-stem alcohol extracts, VTT-leaf hot water extracts or VTT-stem hot water extracts five times per week for 4 weeks. In short-term experiments, the mice were orally received with 100 mg kg-1 of VTT-leaf or stem hot water extracts once for 180 min. The fasting blood was collected for determining the glucose level and lipid profile. The one-way analysis of variance (ANOVA) was used to determine whether there were any statistically significant differences (p<0.05) between the means of three or four independent groups. Results: The alcohol extracts of VTT-stem and leaf were used to examine the hypoglycemic activity in STZ-induced diabetic mice. However, these two kinds of VTT alcohol extracts did not have any effects on blood glucose level of diabetic mice. Second, the hot water extracts of VTT-stem and leaf were orally administered to the STZ-induced diabetic mice for 4 weeks. Interestingly, only hot water extracts of VTT-leaf significantly (p<0.05) decreased blood glucose level, but the stem extracts did not. In the short-term experiments, the blood glucose level of diabetic mice could be quickly decreased after feeding with hot water extracts of VTT-leaf for 2-3 h. In addition, hot water extracts of VTT-leaf could protect pancreatic β-cells from STZ-induced damage by immunohistochemistry staining. On the other hand, only hot water extracts of VTT-stem could decrease triglyceride level in STZ-induced diabetic mice. Conclusion: It is concluded that hot water extracts of VTT-leaf exhibited antidiabetic activity and might be developed as functional food to treat diabetes mellitus in the future.
原文英語
頁(從 - 到)457-464
頁數8
期刊International Journal of Pharmacology
13
發行號5
DOIs
出版狀態已發佈 - 2017

指紋

Experimental Diabetes Mellitus
Vitis
Blood Glucose
Water
Streptozocin
Alcohols
Hypoglycemic Agents
Inbred ICR Mouse
Functional Food
Medicinal Plants
Jaundice
Taiwan
Type 2 Diabetes Mellitus
Hepatitis
Arthritis
Diarrhea
Fasting
Diabetes Mellitus
Analysis of Variance
Triglycerides

ASJC Scopus subject areas

  • Pharmacology

引用此文

Vitis thunbergii var. Taiwaniana leaf extract reduces blood glucose levels in mice with streptozotocin-induced diabetes. / Leung, Wan; Ho, Feng Ming; Li, Wen Pin; Liang, Yu Chih.

於: International Journal of Pharmacology, 卷 13, 編號 5, 2017, p. 457-464.

研究成果: 雜誌貢獻文章

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abstract = "Background and Objective: Vitis thunbergii var. taiwaniana (VTT) is a native folk medicinal plant in Taiwan used to treat hepatitis, jaundice, stomachaches, diarrhea and arthritis. This study aimed to investigate the antidiabetic activity of VTT extracts using a mice model of streptozotocin (STZ)-induced diabetes. Materials and Methods: The ICR male mice were received a single i.p., injection of 100 mg kg-1 of STZ to induce type 2-like diabetes. Diabetic mice were orally administered with 100 mg kg-1 of VTT-leaf alcohol extracts, VTT-stem alcohol extracts, VTT-leaf hot water extracts or VTT-stem hot water extracts five times per week for 4 weeks. In short-term experiments, the mice were orally received with 100 mg kg-1 of VTT-leaf or stem hot water extracts once for 180 min. The fasting blood was collected for determining the glucose level and lipid profile. The one-way analysis of variance (ANOVA) was used to determine whether there were any statistically significant differences (p<0.05) between the means of three or four independent groups. Results: The alcohol extracts of VTT-stem and leaf were used to examine the hypoglycemic activity in STZ-induced diabetic mice. However, these two kinds of VTT alcohol extracts did not have any effects on blood glucose level of diabetic mice. Second, the hot water extracts of VTT-stem and leaf were orally administered to the STZ-induced diabetic mice for 4 weeks. Interestingly, only hot water extracts of VTT-leaf significantly (p<0.05) decreased blood glucose level, but the stem extracts did not. In the short-term experiments, the blood glucose level of diabetic mice could be quickly decreased after feeding with hot water extracts of VTT-leaf for 2-3 h. In addition, hot water extracts of VTT-leaf could protect pancreatic β-cells from STZ-induced damage by immunohistochemistry staining. On the other hand, only hot water extracts of VTT-stem could decrease triglyceride level in STZ-induced diabetic mice. Conclusion: It is concluded that hot water extracts of VTT-leaf exhibited antidiabetic activity and might be developed as functional food to treat diabetes mellitus in the future.",
keywords = "Diabetes mellitus, Glucose, Pancreatic β-cells, Streptozotocin, Vitis thunbergii var. taiwaniana",
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T1 - Vitis thunbergii var. Taiwaniana leaf extract reduces blood glucose levels in mice with streptozotocin-induced diabetes

AU - Leung, Wan

AU - Ho, Feng Ming

AU - Li, Wen Pin

AU - Liang, Yu Chih

PY - 2017

Y1 - 2017

N2 - Background and Objective: Vitis thunbergii var. taiwaniana (VTT) is a native folk medicinal plant in Taiwan used to treat hepatitis, jaundice, stomachaches, diarrhea and arthritis. This study aimed to investigate the antidiabetic activity of VTT extracts using a mice model of streptozotocin (STZ)-induced diabetes. Materials and Methods: The ICR male mice were received a single i.p., injection of 100 mg kg-1 of STZ to induce type 2-like diabetes. Diabetic mice were orally administered with 100 mg kg-1 of VTT-leaf alcohol extracts, VTT-stem alcohol extracts, VTT-leaf hot water extracts or VTT-stem hot water extracts five times per week for 4 weeks. In short-term experiments, the mice were orally received with 100 mg kg-1 of VTT-leaf or stem hot water extracts once for 180 min. The fasting blood was collected for determining the glucose level and lipid profile. The one-way analysis of variance (ANOVA) was used to determine whether there were any statistically significant differences (p<0.05) between the means of three or four independent groups. Results: The alcohol extracts of VTT-stem and leaf were used to examine the hypoglycemic activity in STZ-induced diabetic mice. However, these two kinds of VTT alcohol extracts did not have any effects on blood glucose level of diabetic mice. Second, the hot water extracts of VTT-stem and leaf were orally administered to the STZ-induced diabetic mice for 4 weeks. Interestingly, only hot water extracts of VTT-leaf significantly (p<0.05) decreased blood glucose level, but the stem extracts did not. In the short-term experiments, the blood glucose level of diabetic mice could be quickly decreased after feeding with hot water extracts of VTT-leaf for 2-3 h. In addition, hot water extracts of VTT-leaf could protect pancreatic β-cells from STZ-induced damage by immunohistochemistry staining. On the other hand, only hot water extracts of VTT-stem could decrease triglyceride level in STZ-induced diabetic mice. Conclusion: It is concluded that hot water extracts of VTT-leaf exhibited antidiabetic activity and might be developed as functional food to treat diabetes mellitus in the future.

AB - Background and Objective: Vitis thunbergii var. taiwaniana (VTT) is a native folk medicinal plant in Taiwan used to treat hepatitis, jaundice, stomachaches, diarrhea and arthritis. This study aimed to investigate the antidiabetic activity of VTT extracts using a mice model of streptozotocin (STZ)-induced diabetes. Materials and Methods: The ICR male mice were received a single i.p., injection of 100 mg kg-1 of STZ to induce type 2-like diabetes. Diabetic mice were orally administered with 100 mg kg-1 of VTT-leaf alcohol extracts, VTT-stem alcohol extracts, VTT-leaf hot water extracts or VTT-stem hot water extracts five times per week for 4 weeks. In short-term experiments, the mice were orally received with 100 mg kg-1 of VTT-leaf or stem hot water extracts once for 180 min. The fasting blood was collected for determining the glucose level and lipid profile. The one-way analysis of variance (ANOVA) was used to determine whether there were any statistically significant differences (p<0.05) between the means of three or four independent groups. Results: The alcohol extracts of VTT-stem and leaf were used to examine the hypoglycemic activity in STZ-induced diabetic mice. However, these two kinds of VTT alcohol extracts did not have any effects on blood glucose level of diabetic mice. Second, the hot water extracts of VTT-stem and leaf were orally administered to the STZ-induced diabetic mice for 4 weeks. Interestingly, only hot water extracts of VTT-leaf significantly (p<0.05) decreased blood glucose level, but the stem extracts did not. In the short-term experiments, the blood glucose level of diabetic mice could be quickly decreased after feeding with hot water extracts of VTT-leaf for 2-3 h. In addition, hot water extracts of VTT-leaf could protect pancreatic β-cells from STZ-induced damage by immunohistochemistry staining. On the other hand, only hot water extracts of VTT-stem could decrease triglyceride level in STZ-induced diabetic mice. Conclusion: It is concluded that hot water extracts of VTT-leaf exhibited antidiabetic activity and might be developed as functional food to treat diabetes mellitus in the future.

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KW - Glucose

KW - Pancreatic β-cells

KW - Streptozotocin

KW - Vitis thunbergii var. taiwaniana

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