Visualizing inflammatory activity in isolated perfused rat hearts

Long Sheng Lu, Yen Bin Liu, Chau Chung Wu, Chia Wei Sun

研究成果: 書貢獻/報告類型會議貢獻

摘要

Inflammation is a defense mechanism against various noxious stimuli. The recruitment of host leukocytes to sites of injury results in increased regional microvascular leakage and reactive oxygen species (ROS) generation. Excessive inflammatory activity not only eliminates offending stimuli but also result in tissue damage, as evidenced in reperfusion injury of the heart. To investigate spatial-temporal evolution of acute inflammation after myocardial reperfusion injury, we monitored microvascular leakage and reactive oxygen species generation with optical mapping technique. Reperfusion injury was performed on isolated blood-perfused rat heart, and it was labeled with dihydroethidium and large molecular weight tetramethylrhodamine conjugated dextran. Tissue was illuminated with a 532 nm laser, and epifluorescence at 580 and 650 nm was collected through 2 separate band pass filters. Our results indicate that 1. Optical mapping of myocardial inflammation is feasible; and 2. Reperfusion injury elicits substantial microvascular leakage and ROS production.

原文英語
主出版物標題Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004
頁面150
頁數1
出版狀態已發佈 - 2004
對外發佈Yes
事件Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, 臺灣
持續時間: 十二月 15 2004十二月 17 2004

其他

其他Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004
國家臺灣
城市Taipei
期間12/15/0412/17/04

    指紋

ASJC Scopus subject areas

  • Engineering(all)

引用此

Lu, L. S., Liu, Y. B., Wu, C. C., & Sun, C. W. (2004). Visualizing inflammatory activity in isolated perfused rat hearts. 於 Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 (頁 150)