摘要
Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.
原文 | 英語 |
---|---|
頁(從 - 到) | 446-451 |
頁數 | 6 |
期刊 | Urology |
卷 | 74 |
發行號 | 2 |
DOIs | |
出版狀態 | 已發佈 - 8月 2009 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 泌尿科學