Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model

Kuan Ying Arthur Huang, Chris Ka Fai Li, Elizabeth Clutterbuck, Cecilia Chui, Tom Wilkinson, Anthony Gilbert, John Oxford, Rob Lambkin-Williams, Tzou Yien Lin, Andrew J. Mcmichael, Xiao Ning Xu

研究成果: 雜誌貢獻文章同行評審

36 引文 斯高帕斯(Scopus)

摘要

Background. Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear.Methods. We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus.Results. Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells.Conclusions. Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans.
原文英語
頁(從 - 到)1354-1361
頁數8
期刊Journal of Infectious Diseases
209
發行號9
DOIs
出版狀態已發佈 - 2014
對外發佈

ASJC Scopus subject areas

  • 免疫學和過敏
  • 傳染性疾病

指紋

深入研究「Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model」主題。共同形成了獨特的指紋。

引用此