Vascular endothelial growth factor gene polymorphism and protein expression in the pathogenesis of pterygium

Mei Ling Peng, Yi Yu Tsai, Jai Nien Tung, Chun Chi Chiang, Ying Cher Huang, Huei Lee, Ya Wen Cheng

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

Background and aims Vascular endothelial growth factor (VEGF) gene expression has been linked to cancer progression. Here we hypothesise that the polymorphism and protein expression of VEGF are correlated with the pathogenesis and therapy response of pterygium. Methods 60 pterygial and 121 normal conjunctival samples were collected to determine the genotypes and protein expression of VEGF. Primary pterygium cells (PECs) were used to confirm the effect of the VEGF polymorphism on the angiogenesis of pterygium. Results 48 (83.3%) pterygial specimens tested positive for VEGF protein expression, which was significantly higher than in the control groups (16.7%, pT variant, but not the ?2578C>A variant, was significantly higher in the pterygium group compared with the control group. VEGF protein expression was significantly higher in the 936 C/C group than in the 936 C/T and T/T groups (p=0.001). The results of our cell model showed that PECs with the C/C genotype had a higher angiogenesis ability and higher response to the antiangiogenesis drug bevacizumab than cells with the C/T and T/T genotypes. Conclusions We suggest that VEGF could be used as a target for pterygium therapy in patients with the 936C>T genotype.
原文英語
頁(從 - 到)556-561
頁數6
期刊British Journal of Ophthalmology
98
發行號4
DOIs
出版狀態已發佈 - 2014

指紋

Pterygium
Vascular Endothelial Growth Factor A
Genotype
Proteins
Control Groups
Gene Expression
Therapeutics
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

引用此文

Vascular endothelial growth factor gene polymorphism and protein expression in the pathogenesis of pterygium. / Peng, Mei Ling; Tsai, Yi Yu; Tung, Jai Nien; Chiang, Chun Chi; Huang, Ying Cher; Lee, Huei; Cheng, Ya Wen.

於: British Journal of Ophthalmology, 卷 98, 編號 4, 2014, p. 556-561.

研究成果: 雜誌貢獻文章

Peng, Mei Ling ; Tsai, Yi Yu ; Tung, Jai Nien ; Chiang, Chun Chi ; Huang, Ying Cher ; Lee, Huei ; Cheng, Ya Wen. / Vascular endothelial growth factor gene polymorphism and protein expression in the pathogenesis of pterygium. 於: British Journal of Ophthalmology. 2014 ; 卷 98, 編號 4. 頁 556-561.
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abstract = "Background and aims Vascular endothelial growth factor (VEGF) gene expression has been linked to cancer progression. Here we hypothesise that the polymorphism and protein expression of VEGF are correlated with the pathogenesis and therapy response of pterygium. Methods 60 pterygial and 121 normal conjunctival samples were collected to determine the genotypes and protein expression of VEGF. Primary pterygium cells (PECs) were used to confirm the effect of the VEGF polymorphism on the angiogenesis of pterygium. Results 48 (83.3{\%}) pterygial specimens tested positive for VEGF protein expression, which was significantly higher than in the control groups (16.7{\%}, pT variant, but not the ?2578C>A variant, was significantly higher in the pterygium group compared with the control group. VEGF protein expression was significantly higher in the 936 C/C group than in the 936 C/T and T/T groups (p=0.001). The results of our cell model showed that PECs with the C/C genotype had a higher angiogenesis ability and higher response to the antiangiogenesis drug bevacizumab than cells with the C/T and T/T genotypes. Conclusions We suggest that VEGF could be used as a target for pterygium therapy in patients with the 936C>T genotype.",
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AU - Tsai, Yi Yu

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AU - Huang, Ying Cher

AU - Lee, Huei

AU - Cheng, Ya Wen

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N2 - Background and aims Vascular endothelial growth factor (VEGF) gene expression has been linked to cancer progression. Here we hypothesise that the polymorphism and protein expression of VEGF are correlated with the pathogenesis and therapy response of pterygium. Methods 60 pterygial and 121 normal conjunctival samples were collected to determine the genotypes and protein expression of VEGF. Primary pterygium cells (PECs) were used to confirm the effect of the VEGF polymorphism on the angiogenesis of pterygium. Results 48 (83.3%) pterygial specimens tested positive for VEGF protein expression, which was significantly higher than in the control groups (16.7%, pT variant, but not the ?2578C>A variant, was significantly higher in the pterygium group compared with the control group. VEGF protein expression was significantly higher in the 936 C/C group than in the 936 C/T and T/T groups (p=0.001). The results of our cell model showed that PECs with the C/C genotype had a higher angiogenesis ability and higher response to the antiangiogenesis drug bevacizumab than cells with the C/T and T/T genotypes. Conclusions We suggest that VEGF could be used as a target for pterygium therapy in patients with the 936C>T genotype.

AB - Background and aims Vascular endothelial growth factor (VEGF) gene expression has been linked to cancer progression. Here we hypothesise that the polymorphism and protein expression of VEGF are correlated with the pathogenesis and therapy response of pterygium. Methods 60 pterygial and 121 normal conjunctival samples were collected to determine the genotypes and protein expression of VEGF. Primary pterygium cells (PECs) were used to confirm the effect of the VEGF polymorphism on the angiogenesis of pterygium. Results 48 (83.3%) pterygial specimens tested positive for VEGF protein expression, which was significantly higher than in the control groups (16.7%, pT variant, but not the ?2578C>A variant, was significantly higher in the pterygium group compared with the control group. VEGF protein expression was significantly higher in the 936 C/C group than in the 936 C/T and T/T groups (p=0.001). The results of our cell model showed that PECs with the C/C genotype had a higher angiogenesis ability and higher response to the antiangiogenesis drug bevacizumab than cells with the C/T and T/T genotypes. Conclusions We suggest that VEGF could be used as a target for pterygium therapy in patients with the 936C>T genotype.

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