Vascular Endothelial Growth Factor-C Upregulates Cortactin and Promotes Metastasis of Esophageal Squamous Cell Carcinoma

Chih-Ming Su, Yen-Hao Su, Ching Feng Chiu, Yi Wen Chang, Chih Chen Hong, Yang Hao Yu, Yuan Soon Ho, Chih Hsiung Wu, Chia Sheng Yen, Jen Liang Su

研究成果: 雜誌貢獻文章

13 引文 斯高帕斯(Scopus)

摘要

Background: Vascular endothelial growth factor-C (VEGF-C) plays an important role during cancer progression and metastasis through activation of VEGF receptors. However, the role of VEGF-C in esophageal squamous cell carcinoma (ESCC) remains unclear.

Methods: The expression of VEGF-C in advanced stages of esophageal cancer was examined by immunohistochemistry and its expression was correlated with the protein level of cortactin (CTTN) by Western blot. Knockdown and overexpression of the CTTN protein were respectively performed to investigate the effects on VEGF-C-enhanced ESCC migration/invasion by in vitro transwell assay, cell tracing assay, and tumor growth/experimental metastasis in animal models.

Results: The expression of VEGF-C was positively correlated with tumor status and poor clinical prognosis in patient with esophageal cancer. VEGF-C-upregulated CTTN expression contributed the migration/invasive abilities of ESCC cell lines through Src-mediated downregulation of miR-326. Moreover, knockdown of CTTN expression significantly abolished VEGF-C-induced tumor growth and experimental lung metastasis in vivo.

Conclusions: Upregulation of CTTN is critical for VEGF-C-mediated tumor growth and metastasis of ESCC. These finding suggest that VEGF-C upregulated CTTN expression through Src-mediated downregulation of miR-326. CTTN may be a crucial mediator of VEGF-C-involved ESCC metastasis, which provides a potential target for diagnosis and individualized treatment in clinical practice.
原文英語
頁(從 - 到)767-775
頁數9
期刊Annals of Surgical Oncology
21
發行號4
DOIs
出版狀態已發佈 - 2014

    指紋

ASJC Scopus subject areas

  • Surgery
  • Oncology
  • Medicine(all)

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