Utility of PCR assays for rapid diagnosis of BCG infection in children

W. J. Su, C. Y. Huang, C. Y. Huang, R. P. Perng

研究成果: 雜誌貢獻文章

20 引文 斯高帕斯(Scopus)

摘要

We report Mycobacterium bovis BCG infection in two children vaccinated with BCG (Tokyo strain) on the first day of life. Their diagnoses were made by biopsy of skin lesions and pus from an anterior chest wall abscess, respectively, yielding a positive culture of mycobacteria fully susceptible to rifampicin, isoniazid and ethambutol, but resistant to pyrazinamide. M. bovis BCG was identified by a negative niacin test, absence of nitrate reductase and resistance to pyrazinamide and cycloserine. The diagnoses were further confirmed by a combination of an allele-specific polymerase chain reaction (PCR) and a multiplex PCR method. Based on the drug susceptibility results, treatment with rifampicin, isoniazid and ethambutol was instituted. One patient (Case 1) improved clinically and is well after treatment. However, the other patient with severe combined immunodeficiency died of disseminated BCG infection in spite of intensive anti-tuberculosis therapy. Although BCG is considered to be a safe vaccine, it should be kept in mind that complications related to BCG do occur.

原文英語
頁(從 - 到)380-384
頁數5
期刊International Journal of Tuberculosis and Lung Disease
5
發行號4
出版狀態已發佈 - 2001
對外發佈Yes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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