Using a hybrid radioenhancer to discover tumor cell-targeted treatment for osteosarcoma: An in vitro study

Fu I. Tung, Li Chin Chen, Yu Chi Wang, Ming Hong Chen, Pei Wei Shueng, Tse Ying Liu

研究成果: 雜誌貢獻文章同行評審

摘要

Osteosarcoma is insensitive to radiation. High-dose radiation is often used as a treatment but causes side effects in patients. Hence, it is important to develop tumor cell-targeted radiotherapy that could improve radiotherapy efficiency on tumor cells and reduce the toxic effect on normal cells during radiation treatment. In this study, we developed an innovative method for treating osteosarcoma by using a novel radiation-enhancer (i.e., carboxymethyl-hexanoyl chitosan-coated self-assembled Au@Fe3O4 nanoparticles; CSAF NPs). CSAF NPs were employed together with 5-aminolevulinic acid (5ALA) to achieve tumor cell-targeted radiotherapy. In this study, osteosarcoma cells (MG63) and normal cells (MC3T3-E1) were used for an in vitro investigation, in which reactive oxygen species (ROS) assay, cell viability assay, clonogenic assay, and western blot were used to confirm the treatment efficiency. The ROS assay showed that the combination of CSAF NPs and 5-ALA enhanced radiation-induced ROS production in tumor cells (MG63); however, this was not observed in normal cells (MC3T3-E1). The cell viability ratio of normal cells to tumor cells after treatment with CSAF NPs and 5-ALA reached 2.79. Moreover, the clonogenic assay showed that the radiosensitivity of MG63 cells was increased by the combination use of CSAF NPs and 5-ALA. This was supported by performing a western blot that confirmed the expression of cytochrome c (a marker of cell mitochondria damage) and caspase-3 (a marker of cell apoptosis). The results provide an essential basis for developing tumor-cell targeted radiotherapy by means of low-dose radiation.

原文英語
頁(從 - 到)3877-3889
頁數13
期刊Current Medicinal Chemistry
28
發行號19
DOIs
出版狀態已發佈 - 2021

ASJC Scopus subject areas

  • 生物化學
  • 分子醫學
  • 藥理
  • 藥物發現
  • 有機化學

指紋

深入研究「Using a hybrid radioenhancer to discover tumor cell-targeted treatment for osteosarcoma: An in vitro study」主題。共同形成了獨特的指紋。

引用此