Use of wogonin as a cooperative drug with praziquantel to better combat schistosomiasis

Ho Yin Pekkle Lam, Meng Yun Hung, Po Ching Cheng, Shih Yi Peng

研究成果: 雜誌貢獻文章同行評審

摘要

Background: Schistosomiasis is one of the most devastating tropical diseases in the world. Currently, praziquantel (PZQ) represents the best pharmacological option for the treatment of schistosomiasis as it effectively kills the worm. However, the inability to reverse established liver damages often makes treatment futile. In the current study, we investigate whether combining the use of wogonin, a compound that was found to be liver-protective, with PZQ can attribute to the greatest beneficial effect in Schistosoma mansoni-infected mice. Methods: To determine the protective effect of PZQ-wogonin treatment on S. manosni-infected mice, histopathological analysis was done to evaluate the granuloma size and fibrotic areas in the liver. Western blotting was performed to analyze several injuries-related markers including fibrotic markers, inflammasomes, and apoptotic markers. Scanning electron microscopy was done to evaluate the effect of wogonin on the worms, and the worm and egg burden was calculated. Results: Our results showed that PZQ-wogonin treatment significantly improved liver histopathology of S. mansoni-infected mice. Further analysis showed that PZQ-wogonin combinations are more effective in reducing fibrosis, inflammation, and apoptosis in the liver than that of individual drug use. Furthermore, our results revealed that wogonin is anthelmintic; and it works better with PZQ in reducing hepatic egg burden, further lessen the disease progression. Conclusion: In general, this combinatorial strategy may represent a new and effective approach to schistosomiasis treatment.
原文英語
頁(從 - 到)757-765
頁數9
期刊Journal of Microbiology, Immunology and Infection
55
發行號4
DOIs
出版狀態已發佈 - 8月 2022

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學與微生物學 (全部)
  • 微生物學(醫學)
  • 傳染性疾病

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