Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure

Chao Yuan Huang, Chien Tien Su, Chi Jung Chung, Yeong Shiau Pu, Jan Show Chu, Hsiu Yuan Yang, Chia Chang Wu, Yu Mei Hsueh

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case–control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC–MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose–response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p = 0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC.
原文英語
頁(從 - 到)349-354
頁數6
期刊Toxicology and Applied Pharmacology
262
發行號3
DOIs
出版狀態已發佈 - 八月 1 2012

指紋

Arsenic
Renal Cell Carcinoma
Cells
Odds Ratio
Cacodylic Acid
Image-Guided Biopsy
Atomic absorption spectrometry
Biopsy
Liquid chromatography
High performance liquid chromatography
8-oxo-7-hydrodeoxyguanosine
Hydrides
Tandem Mass Spectrometry
Mass spectrometry
Tumors
Liquid Chromatography
DNA Damage
Case-Control Studies
Spectrum Analysis
High Pressure Liquid Chromatography

Keywords

  • Arsenic
  • 8-Hydroxydeoxyguanosine 5′-monophosphate
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

引用此文

Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure. / Huang, Chao Yuan; Su, Chien Tien; Chung, Chi Jung; Pu, Yeong Shiau; Chu, Jan Show; Yang, Hsiu Yuan; Wu, Chia Chang; Hsueh, Yu Mei.

於: Toxicology and Applied Pharmacology, 卷 262, 編號 3, 01.08.2012, p. 349-354.

研究成果: 雜誌貢獻文章

@article{c473d7f7f8ca4827b15693304af031f5,
title = "Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure",
abstract = "8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case-control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose-response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p=0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC.",
keywords = "8-Hydroxydeoxyguanosine 5'-monophosphate, Arsenic, Renal cell carcinoma, Arsenic, 8-Hydroxydeoxyguanosine 5′-monophosphate, Renal cell carcinoma",
author = "Huang, {Chao Yuan} and Su, {Chien Tien} and Chung, {Chi Jung} and Pu, {Yeong Shiau} and Chu, {Jan Show} and Yang, {Hsiu Yuan} and Wu, {Chia Chang} and Hsueh, {Yu Mei}",
year = "2012",
month = "8",
day = "1",
doi = "10.1016/j.taap.2012.05.013",
language = "English",
volume = "262",
pages = "349--354",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure

AU - Huang, Chao Yuan

AU - Su, Chien Tien

AU - Chung, Chi Jung

AU - Pu, Yeong Shiau

AU - Chu, Jan Show

AU - Yang, Hsiu Yuan

AU - Wu, Chia Chang

AU - Hsueh, Yu Mei

PY - 2012/8/1

Y1 - 2012/8/1

N2 - 8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case-control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose-response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p=0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC.

AB - 8-Hydroxydeoxyguanosine (8-OHdG) is one of the most reliable and abundant markers of DNA damage. The study was designed to explore the relationship between urinary 8-OHdG and renal cell carcinoma (RCC) and to investigate whether individuals with a high level of 8-OHdG would have a modified odds ratio (OR) of arsenic-related RCC. This case-control study was conducted with 132 RCC patients and 245 age- and sex-matched controls from a hospital-based pool between November 2006 and May 2009. Pathological verification of RCC was completed by image-guided biopsy or surgical resection of renal tumors. Urinary 8-OHdG levels were determined using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Concentrations of urinary arsenic species, including inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA), were determined by a high performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. Level of urinary 8-OHdG was significantly associated with the OR of RCC in a dose-response relationship after multivariate adjustment. Urinary 8-OHdG was significantly related to urinary total arsenic. The greatest OR (3.50) was seen in the individuals with high urinary 8-OHdG and high urinary total arsenic. A trend test indicated that the OR of RCC was increased with one of these factors and was further increased with both (p=0.002). In conclusion, higher urinary 8-OHdG was a strong predictor of the RCC. High levels of 8-OHdG combined with urinary total arsenic might be indicative of arsenic-induced RCC.

KW - 8-Hydroxydeoxyguanosine 5'-monophosphate

KW - Arsenic

KW - Renal cell carcinoma

KW - Arsenic

KW - 8-Hydroxydeoxyguanosine 5′-monophosphate

KW - Renal cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=84863853447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863853447&partnerID=8YFLogxK

U2 - 10.1016/j.taap.2012.05.013

DO - 10.1016/j.taap.2012.05.013

M3 - Article

C2 - 22659136

AN - SCOPUS:84863853447

VL - 262

SP - 349

EP - 354

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 3

ER -