Urinary proteome and potential biomarkers associated with serial pathogenesis steps of focal segmental glomerulosclerosis

Hao Ai Shui, Tzu Hao Huang, Shuk Man Ka, Pei Hsiu Chen, Yuh Feng Lin, Ann Chen

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)

摘要

Background. Focal segmental glomerulosclerosis (FSGS) is a chronic nephropathy showing characteristic glomerular sclerosis. So far, the diagnosis and prognosis of FSGS rely mainly on the invasive biopsy. Searching for potential FSGS-associated urinary biomarkers representing pre-sclerotic and serial sclerotic stages of FSGS could be helpful to the non-invasive diagnosis and prognosis of FSGS. Methods. In the present study, we used a 2D gel-based proteomic approach to identify urinary proteins at pre-sclerotic and different sclerotic stages of an FSGS mouse model in order to find FSGS-related urinary proteins. The FSGS mouse model was established in Balb/c mice by a single injection of adriamycin, and disease severity was monitored by renal biological parameters and histopathological features. Urine was collected on days 0, 4, 7, 11, 15 and 20, and subjected to two-dimensional electrophoresis (2-DE) analysis. Proteins were identified by matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) and a protein database search. Some of the identified proteins were confirmed by western blot analysis. Results. We identified 37 urinary proteins showing characteristic patterns of dynamic changes along the disease course of FSGS. Early urinary proteins appearing before glomerular scleoris were noticed. Importantly, 11 urine proteins are novel to FSGS and have known functions highly associated with different pathogenetic steps of the disease, including haemodynamic disturbance, podocyte apoptosis, ECM-protein deposition and glomerular sclerosis. Conclusions. Some urinary proteins appearing earlier than glomerular sclerosis could serve as potential early diagnostic biomarkers. The proteins with the pathogenic roles could serve as potential non-invasive prognostic markers of FSGS, and give an insight into pathogenic mechanisms of this sclerosis disease.

原文英語
頁(從 - 到)176-185
頁數10
期刊Nephrology Dialysis Transplantation
23
發行號1
DOIs
出版狀態已發佈 - 一月 2008
對外發佈

ASJC Scopus subject areas

  • 腎臟病學
  • 移植

指紋

深入研究「Urinary proteome and potential biomarkers associated with serial pathogenesis steps of focal segmental glomerulosclerosis」主題。共同形成了獨特的指紋。

引用此