Ultrastructural changes of bladder cancer cells following methylene blue-sensitized photodynamic treatment

D. S. Yu, S. Y. Chang, C. P. Ma

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

The cytotoxic efficacy of methylene blue-sensitized photoinactivation of bladder cancer cells has been proved in vitro and in vivo in our previous work. The ultrastructural changes of MBT-2 murine bladder cancer cells following methylene blue-sensitized phototherapy have been evaluated by scanning and transmission electron microscopy. MBT-2 cells were pretreated with 0.1% methylene blue, and then exposed to ordinary light for different durations from 1 to 60 min. Both electron microscopic examinations showed that changes in tumor cells occurred at 5 min of illumination with fenestration and minipore formation on the cell membrane and mitochondrial disintegration. At 30 min, cleavage and cracking of the cell membrane with ribosomal lysis were noted. Finally, fragmentation with decomposition of the membrane and chromosomal condensation and lysis resulted at 60 min. This study showed that the cytotoxic effect induced by methylene blue phototherapy is initiated in the cell membrane and intracellular mitochondria in the early phase.

原文英語
頁(從 - 到)322-326
頁數5
期刊European Urology
19
發行號4
DOIs
出版狀態已發佈 - 一月 1 1991
對外發佈Yes

指紋

Methylene Blue
Urinary Bladder Neoplasms
Phototherapy
Cell Membrane
Scanning Transmission Electron Microscopy
Therapeutics
Lighting
Mitochondria
Electrons
Light
Membranes
Neoplasms

ASJC Scopus subject areas

  • Urology

引用此文

Ultrastructural changes of bladder cancer cells following methylene blue-sensitized photodynamic treatment. / Yu, D. S.; Chang, S. Y.; Ma, C. P.

於: European Urology, 卷 19, 編號 4, 01.01.1991, p. 322-326.

研究成果: 雜誌貢獻文章

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