Ultra sensitive detection of eneterovirus 71 by modified electrochemical impedance spectroscopy

Shin Hua Tseng, Dion T. Tseng, Tzu Cheng Lee, Tsai Mu Cheng, Jyh Yuan Yang, Ruo Yu Hsieh, Chuan Mei Tsai, Chia Ching Chang

研究成果: 書貢獻/報告類型會議貢獻

摘要

The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
原文英語
主出版物標題Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
頁面27-28
頁數2
出版狀態已發佈 - 2010
對外發佈Yes
事件1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, 美国
持續時間: 二月 7 2010二月 10 2010

其他

其他1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
國家美国
城市Houston, TX
期間2/7/102/10/10

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

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