Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia

Chih Ming Ho, Bor Heng Lee, Shwu Fen Chang, Tsai Yen Chien, Shih Hung Huang, Chiu Cho Yan, Wen Fang Cheng

研究成果: 雜誌貢獻文章同行評審

26 引文 斯高帕斯(Scopus)

摘要

This study aimed to evaluate whether quantitation of high-risk human papillomavirus (HR-HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/ GP6+ PCR for HPV DNA amplification, followed by HPV genotype-specific hybridization with on a gene chip. E6 type-specific PCR was used to validate multiple infections. Quantitative real-time reverse transcriptase (QRT-PCR) and real-time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid-based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA-positive cases was lower in normal women and

原文英語
頁(從 - 到)622-632
頁數11
期刊International Journal of Cancer
127
發行號3
DOIs
出版狀態已發佈 - 八月 1 2010

ASJC Scopus subject areas

  • 癌症研究
  • 腫瘤科

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