Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells

C. W. Chen, Yu-Hui Tsai, W. P. Deng, S. N. Shih, C. L. Fang, J. G. Burch, W. H. Chen, Wen-FuThomas Lai

研究成果: 雜誌貢獻文章同行評審

80 引文 斯高帕斯(Scopus)

摘要

Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor-beta 1 (TGF-β1) and dexamethasone. Extracellular matrix (ECM) also regulates the differentiation by MPCs. To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. That model exposed to dexamethasone, recombinant human TGF-β1(rhTGF-β1) and collagens. The results showed that MPCs incorporated with dexamethasone and rhTGF-β1 increased proliferation and expression of glycosaminoglycan (GAG) after 14 days. Type II collagen enhanced the GAG synthesis, but did not increase alkaline phosphatase (ALP) activity. When adding dexamethasone and rhTGF-β1 MPCs increased mRNA expression of Sox9. Incorporation with type II collagen, dexamethasone and rhTGF-β1, MPCs induced mRNA expression of aggrecan and enhanced levels of type II collagen, and Sox9 mRNA. In contrast, incorporation with type I collagen, dexamethasone and rhTGF-β1 MPCs reduced levels of aggrecan, and Sox9 mRNA, and showed no type II collagen mRNA. Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs.
原文英語
頁(從 - 到)446-453
頁數8
期刊Journal of Orthopaedic Research
23
發行號2
DOIs
出版狀態已發佈 - 三月 2005

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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