The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.
|頁（從 - 到）||353-357|
|期刊||Proceedings of the National Academy of Sciences of the United States of America|
|出版狀態||已發佈 - 一月 1 1983|
ASJC Scopus subject areas
Fung, Y. K. T., Crittenden, L. B., Fadly, A. M., & Kung, H. J. (1983). Tumor induction by direct injection of cloned v-src DNA into chickens. Proceedings of the National Academy of Sciences of the United States of America, 80(2 I), 353-357.