Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis

I. Lun Hsiao, Yi Kong Hsieh, Chu Fang Wang, I. Chieh Chen, Yuh Jeen Huang

研究成果: 雜誌貢獻文章

84 引文 (Scopus)

摘要

The so-called "Trojan-horse" mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior in the cellular uptake of Ag nanoparticles (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs and Ag ions (I) in cells, it cannot be fully proven without examining those two components in both intra- and extracellular media. In our study, we found that even though cells take up AgNPs similarly to (microglia (BV-2)) or more rapidly than (astrocyte (ALT)) Ag (I), the ratio of AgNPs to total Ag (AgNPs+Ag (I)) in both cells was lower than that in outside media. It could be explained that H2O2, a major intracellular reactive oxygen species (ROS), reacts with AgNPs to form more Ag (I). Moreover, the major speciation of Ag (I) in cells was Ag(cysteine) and Ag(cysteine)2, indicating the possible binding of monomer cysteine or vital thiol proteins/peptides to Ag ions. Evidence we found indicates that the Trojan-horse mechanism really exists.
原文英語
頁(從 - 到)3813-3821
頁數9
期刊Environmental Science and Technology
49
發行號6
DOIs
出版狀態已發佈 - 三月 17 2015
對外發佈Yes

指紋

Silver
silver
Nanoparticles
Cysteine
Ions
ion
thiol
Poisons
nanoparticle
analysis
Sulfhydryl Compounds
peptide
Reactive Oxygen Species
Monomers
Peptides
protein
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

引用此文

Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis. / Hsiao, I. Lun; Hsieh, Yi Kong; Wang, Chu Fang; Chen, I. Chieh; Huang, Yuh Jeen.

於: Environmental Science and Technology, 卷 49, 編號 6, 17.03.2015, p. 3813-3821.

研究成果: 雜誌貢獻文章

@article{6a32f46953114493a859aaa160d637e7,
title = "Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis",
abstract = "The so-called {"}Trojan-horse{"} mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior in the cellular uptake of Ag nanoparticles (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs and Ag ions (I) in cells, it cannot be fully proven without examining those two components in both intra- and extracellular media. In our study, we found that even though cells take up AgNPs similarly to (microglia (BV-2)) or more rapidly than (astrocyte (ALT)) Ag (I), the ratio of AgNPs to total Ag (AgNPs+Ag (I)) in both cells was lower than that in outside media. It could be explained that H2O2, a major intracellular reactive oxygen species (ROS), reacts with AgNPs to form more Ag (I). Moreover, the major speciation of Ag (I) in cells was Ag(cysteine) and Ag(cysteine)2, indicating the possible binding of monomer cysteine or vital thiol proteins/peptides to Ag ions. Evidence we found indicates that the Trojan-horse mechanism really exists.",
author = "Hsiao, {I. Lun} and Hsieh, {Yi Kong} and Wang, {Chu Fang} and Chen, {I. Chieh} and Huang, {Yuh Jeen}",
year = "2015",
month = "3",
day = "17",
doi = "10.1021/es504705p",
language = "English",
volume = "49",
pages = "3813--3821",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis

AU - Hsiao, I. Lun

AU - Hsieh, Yi Kong

AU - Wang, Chu Fang

AU - Chen, I. Chieh

AU - Huang, Yuh Jeen

PY - 2015/3/17

Y1 - 2015/3/17

N2 - The so-called "Trojan-horse" mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior in the cellular uptake of Ag nanoparticles (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs and Ag ions (I) in cells, it cannot be fully proven without examining those two components in both intra- and extracellular media. In our study, we found that even though cells take up AgNPs similarly to (microglia (BV-2)) or more rapidly than (astrocyte (ALT)) Ag (I), the ratio of AgNPs to total Ag (AgNPs+Ag (I)) in both cells was lower than that in outside media. It could be explained that H2O2, a major intracellular reactive oxygen species (ROS), reacts with AgNPs to form more Ag (I). Moreover, the major speciation of Ag (I) in cells was Ag(cysteine) and Ag(cysteine)2, indicating the possible binding of monomer cysteine or vital thiol proteins/peptides to Ag ions. Evidence we found indicates that the Trojan-horse mechanism really exists.

AB - The so-called "Trojan-horse" mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior in the cellular uptake of Ag nanoparticles (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs and Ag ions (I) in cells, it cannot be fully proven without examining those two components in both intra- and extracellular media. In our study, we found that even though cells take up AgNPs similarly to (microglia (BV-2)) or more rapidly than (astrocyte (ALT)) Ag (I), the ratio of AgNPs to total Ag (AgNPs+Ag (I)) in both cells was lower than that in outside media. It could be explained that H2O2, a major intracellular reactive oxygen species (ROS), reacts with AgNPs to form more Ag (I). Moreover, the major speciation of Ag (I) in cells was Ag(cysteine) and Ag(cysteine)2, indicating the possible binding of monomer cysteine or vital thiol proteins/peptides to Ag ions. Evidence we found indicates that the Trojan-horse mechanism really exists.

UR - http://www.scopus.com/inward/record.url?scp=84925012646&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925012646&partnerID=8YFLogxK

U2 - 10.1021/es504705p

DO - 10.1021/es504705p

M3 - Article

C2 - 25692749

AN - SCOPUS:84925012646

VL - 49

SP - 3813

EP - 3821

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 6

ER -