Trojan-horse mechanism in the cellular uptake of silver nanoparticles verified by direct intra- and extracellular silver speciation analysis

I. Lun Hsiao, Yi Kong Hsieh, Chu Fang Wang, I. Chieh Chen, Yuh Jeen Huang

研究成果: 雜誌貢獻文章

103 引文 斯高帕斯(Scopus)

摘要

The so-called "Trojan-horse" mechanism, in which nanoparticles are internalized within cells and then release high levels of toxic ions, has been proposed as a behavior in the cellular uptake of Ag nanoparticles (AgNPs). While several reports claim to have proved this mechanism by measuring AgNPs and Ag ions (I) in cells, it cannot be fully proven without examining those two components in both intra- and extracellular media. In our study, we found that even though cells take up AgNPs similarly to (microglia (BV-2)) or more rapidly than (astrocyte (ALT)) Ag (I), the ratio of AgNPs to total Ag (AgNPs+Ag (I)) in both cells was lower than that in outside media. It could be explained that H2O2, a major intracellular reactive oxygen species (ROS), reacts with AgNPs to form more Ag (I). Moreover, the major speciation of Ag (I) in cells was Ag(cysteine) and Ag(cysteine)2, indicating the possible binding of monomer cysteine or vital thiol proteins/peptides to Ag ions. Evidence we found indicates that the Trojan-horse mechanism really exists.
原文英語
頁(從 - 到)3813-3821
頁數9
期刊Environmental Science and Technology
49
發行號6
DOIs
出版狀態已發佈 - 三月 17 2015
對外發佈Yes

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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