Tripolyphosphate cross-linked macromolecular composites for the growth of shape- and size-controlled apatites

Shu Huei Yu, Shao Jung Wu, Jui Yu Wu, Chih Kang Peng, Fwu Long Mi

研究成果: 雜誌貢獻文章

14 引文 斯高帕斯(Scopus)

摘要

Bioactive composites that enable the formation of calcium phosphates have received increased attention over the last decade, in the development of osteoconductive biomaterials for orthopaedic applications. In this work, tripolyphosphate (TPP)-cross-linked chitosan/gelatin composites (TPP-CG) were prepared for the growth of shape- and size-controlled calcium phosphates on/in the composites. The mineralization pattern of the composites, after soaking in the Ca(OH)2 aqueous solution, clearly demonstrated oriented, needle-like nanocrystallites of calcium phosphates in the matrix with especially high Ca/P molar ratio (3.98) as detected by energy dispersive X-ray spectroscopy (EDX) analysis. Subsequent to mineralization in a simulated body fluid (SBF), the mineralized composites showed micro-scaled spherical aggregates deposited on the surface and granule-like nanocrystallites grew in the matrix. The Ca/P molar ratio (1.72) and X-ray diffraction pattern of the nanocrystallites grown in the composites were similar to those of hydroxyapatite (HAp). Osteoblastic differentiation of ROS cells cultured on the mineralized composites allowed an enhanced expression of the chosen osteogenic marker (alkaline phosphatase, ALPase). These results indicated that the composites mineralized with micro- and nano-scaled calcium phosphates with various structural features make them attractive for bone tissue engineering applications.

原文英語
頁(從 - 到)27-40
頁數14
期刊Molecules
18
發行號1
DOIs
出版狀態已發佈 - 一月 2013

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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