Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element

Anthony A.G. Ridgway, Hsing Jien Kung, Donald J. Fujita

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.
原文英語
頁(從 - 到)3199-3215
頁數17
期刊Nucleic Acids Research
17
發行號8
DOIs
出版狀態已發佈 - 四月 25 1989
對外發佈Yes

ASJC Scopus subject areas

  • Genetics

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