Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization

Ankita Kothari, Marimikel Charrier, Yu Wei Wu, Stephanie Malfatti, Carol E. Zhou, Steven W. Singer, Larry Dugan, Aindrila Mukhopadhyay

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

The hydrocarbonoclastic bacterium Acinetobacter venetianus RAG-1 has attracted substantial attention due to its powerful oil-degrading capabilities and its potential to play an important ecological role in the cleanup of alkanes. In this study, we compare the transcriptome of the strain RAG-1 grown in dodecane, the corresponding alkanol (dodecanol), and sodium acetate for the characterization of genes involved in dodecane uptake and utilization. Comparison of the transcriptional responses of RAG-1 grown on dodecane led to the identification of 1074 genes that were differentially expressed relative to sodium acetate. Of these, 622 genes were upregulated when grown in dodecane. The highly upregulated genes were involved in alkane catabolism, along with stress response. Our data suggest AlkMb to be primarily involved in dodecane oxidation. Transcriptional response of RAG-1 grown on dodecane relative to dodecanol also led to the identification of permease, outer membrane protein and thin fimbriae coding genes potentially involved in dodecane uptake. This study provides the first model for key genes involved in alkane uptake and metabolism in A. venetianus RAG-1.
原文英語
文章編號fnw224
期刊FEMS Microbiology Letters
363
發行號20
DOIs
出版狀態已發佈 - 2016

指紋

RAG-1 Genes
Acinetobacter
Oils
Bacteria
Alkanes
Genes
Sodium Acetate
Membrane Transport Proteins
n-dodecane
Transcriptome
Membrane Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

引用此文

Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization. / Kothari, Ankita; Charrier, Marimikel; Wu, Yu Wei; Malfatti, Stephanie; Zhou, Carol E.; Singer, Steven W.; Dugan, Larry; Mukhopadhyay, Aindrila.

於: FEMS Microbiology Letters, 卷 363, 編號 20, fnw224, 2016.

研究成果: 雜誌貢獻文章

Kothari, Ankita ; Charrier, Marimikel ; Wu, Yu Wei ; Malfatti, Stephanie ; Zhou, Carol E. ; Singer, Steven W. ; Dugan, Larry ; Mukhopadhyay, Aindrila. / Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization. 於: FEMS Microbiology Letters. 2016 ; 卷 363, 編號 20.
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abstract = "The hydrocarbonoclastic bacterium Acinetobacter venetianus RAG-1 has attracted substantial attention due to its powerful oil-degrading capabilities and its potential to play an important ecological role in the cleanup of alkanes. In this study, we compare the transcriptome of the strain RAG-1 grown in dodecane, the corresponding alkanol (dodecanol), and sodium acetate for the characterization of genes involved in dodecane uptake and utilization. Comparison of the transcriptional responses of RAG-1 grown on dodecane led to the identification of 1074 genes that were differentially expressed relative to sodium acetate. Of these, 622 genes were upregulated when grown in dodecane. The highly upregulated genes were involved in alkane catabolism, along with stress response. Our data suggest AlkMb to be primarily involved in dodecane oxidation. Transcriptional response of RAG-1 grown on dodecane relative to dodecanol also led to the identification of permease, outer membrane protein and thin fimbriae coding genes potentially involved in dodecane uptake. This study provides the first model for key genes involved in alkane uptake and metabolism in A. venetianus RAG-1.",
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