Transcriptome analysis in blastocyst hatching by cDNA microarray

Huei Wen Chen, Jeremy J W Chen, Sung Liang Yu, Han Ni Li, Pan Chyr Yang, Ching Mao Su, Heng Kien Au, Ching-Wen Chang, Li Wei Chien, Chieh Sheng Chen, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章

27 引文 斯高帕斯(Scopus)

摘要

Background: Hatching is an important process for early embryo development, differentiation and implantation. However, little is known about its regulatory mechanisms. By integrating the technologies of RNA amplification and cDNA microarrays, it has become possible to study the gene expression profile at this critical stage. Methods: Pre-hatched and hatched ICR mouse embryos (25 blastocysts in each group were used in the triplicate experiments) were collected for RNA extraction, amplification, and microarray analysis (the mouse cDNA microarray, 6144 genes, including expressed sequence tags). Results: According to cDNA microarray data, we have identified 85 genes that were expressed at a higher level in hatched blastocyst than in pre-hatched blastocysts. In this study, 47 hatching-related candidate genes were verified via re-sequencing. Some of these genes have been selected and confirmed by real-time quantitative RT-PCR. These hatching-specific genes were also expressed at a lower level in the delayed growth embryos (morula or blastocyst without hatching at day 6 post hCG). These genes included: cell adhesion and migration molecules [E-cadherin, neuronal cell adhesion molecule (NCAM), lectin, galactose binding, soluble 7 (Lgals7), vanin 3 and biglycan], epigenetic regulators (Dnmt1, and SIN3 yeast homolog A), stress response regulators (heme oxygenase 1) and immunoresponse regulators [interleukin (IL)-2-inducible T-cell kinase, IL-4R, interferon- γ receptor 2, and neurotrophin]. The immunostaining of E-cadherin and NCAM showed strong and specific localization in hatched blastocyst. Conclusions: This work provides important information for studying the mechanisms of blastocyst hatching and implantation. These hatching-specific genes may have potential as new drug targets for controlling fertility.

原文英語
頁(從 - 到)2492-2501
頁數10
期刊Human Reproduction
20
發行號9
DOIs
出版狀態已發佈 - 九月 2005

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

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  • 引用此

    Chen, H. W., Chen, J. J. W., Yu, S. L., Li, H. N., Yang, P. C., Su, C. M., Au, H. K., Chang, C-W., Chien, L. W., Chen, C. S., & Tzeng, C. R. (2005). Transcriptome analysis in blastocyst hatching by cDNA microarray. Human Reproduction, 20(9), 2492-2501. https://doi.org/10.1093/humrep/dei084