Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus

M. F. Tsai, C. F. Lo, M. C W Van Hulten, H. F. Tzeng, C. M. Chou, C. J. Huang, C. H. Wang, J. Y. Lin, J. M. Vlak, G. H. Kou

研究成果: 雜誌貢獻文章同行評審

71 引文 斯高帕斯(Scopus)

摘要

The causative agent of white spot syndrome (WSS) is a large double-stranded DNA virus, WSSV, which is probably a representative of a new genus, provisionally called Whispovirus. From previously constructed WSSV genomic libraries of a Taiwan WSSV isolate, clones with open reading frames (ORFs) that encode proteins with significant homology to the class I ribonucleotide reductase large (RR1) and small (RR2) subunits were identified. WSSV rr1 and rr2 potentially encode 848 and 413 amino acids, respectively. RNA was isolated from WSSV-infected shrimp at different times after infection and Northern blot analysis with rr1- and rr2-specific riboprobes found major transcripts of 2.8 and 1.4 kb, respectively. 5' RACE showed that the major rr1 transcript started at a position of -84 (C) relative to the ATG translational start, while transcription of the rr2 gene started at nucleotide residue -68 (T). A consensus motif containing the transcriptional start sites for rr1 and rr2 was observed (TCAc/tTC). Northern blotting and RT-PCR showed that the transcription of rr1 and rr2 started 4-6 h after infection and continued for at least 60 h. The rr1 and rr2 genes thus appear to be WSSV 'early genes'. (C) 2000 Academic Press.
原文英語
頁(從 - 到)92-99
頁數8
期刊Virology
277
發行號1
DOIs
出版狀態已發佈 - 十一月 10 2000
對外發佈

ASJC Scopus subject areas

  • 病毒學
  • 傳染性疾病

指紋

深入研究「Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus」主題。共同形成了獨特的指紋。

引用此