Transcription-driven supercoiling of DNA: Direct biochemical evidence from in vitro studies

Yeou Ping Tsao, Hai Young Wu, Leroy-Fong Liu

研究成果: 雜誌貢獻文章同行評審

247 引文 斯高帕斯(Scopus)

摘要

The translocation of an RNA polymerase elongation complex along double helical DNA has been propose to generate positive supercoiling waves ahead of and negative supercoiling waves behind the transcription ensemble. This twin supercoiled domain model has been tested in vitro. In the presence of prokaryotic DNA topoisomerase I, which selectively removes negative supercoils, transcription from a single promoter results in rapid and extensive positive supercoiling of the DNA template. The accumulation of positive supercoils in the DNA template requires continued movement of the elongation complex as well as sizable nascent RNA chains. These in vitro results provide direct biochemical evidence supporting the twin supercoiled domain model of transcription. Furthermore, the magnitude of DNA supercoiling (torsional) waves generated by transcription is much greater than previously expected, suggesting that transcription is one of the principal affecting intracellular DNA supercoiling.

原文英語
頁(從 - 到)111-118
頁數8
期刊Cell
56
發行號1
DOIs
出版狀態已發佈 - 一月 13 1989
對外發佈Yes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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