Transcription-dependent degradation of topoisomerase I-DNA covalent complexes

Shyamal D. Desai, Hui Zhang, Alexandra Rodriguez-Bauman, Jin Ming Yang, Xiaohua Wu, Murugesan K. Gounder, Eric H. Rubin, Leroy F. Liu

研究成果: 雜誌貢獻文章同行評審

112 引文 斯高帕斯(Scopus)

摘要

Topoisomerase I (Top I)-DNA covalent complexes represent a unique type of DNA lesion whose repair and processing remain unclear. In this study, we show that Top I-DNA covalent complexes transiently arrest RNA transcription in normal nontransformed cells. Arrest of RNA transcription is coupled to activation of proteasomal degradation of Top I and the large subunit of RNA polymerase II. Recovery of transcription occurs gradually and depends on both proteasomal degradation of Top I and functional transcription-coupled repair (TCR). These results suggest that arrest of the RNA polymerase elongation complex by the Top I-DNA covalent complex triggers a 26S proteasome-mediated signaling pathway(s) leading to degradation of both Top I and the large subunit of RNA polymerase II. We propose that proteasomal degradation of Top I and RNA polymerase II precedes repair of the exposed single-strand breaks by TCR.

原文英語
頁(從 - 到)2341-2350
頁數10
期刊Molecular and Cellular Biology
23
發行號7
DOIs
出版狀態已發佈 - 四月 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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