Topoisomerase II is a structural component of mitotic chromosome scaffolds

William C. Earnshaw, Brian Halligan, Carol A. Cooke, Margarete M S Heck, Leroy-Fong Liu

研究成果: 雜誌貢獻文章

574 引文 斯高帕斯(Scopus)

摘要

We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.

原文英語
頁(從 - 到)1706-1715
頁數10
期刊Journal of Cell Biology
100
發行號5
DOIs
出版狀態已發佈 - 五月 1 1985
對外發佈Yes

ASJC Scopus subject areas

  • Cell Biology

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