Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Rong Sen Yang, Huei Ping Tzeng, Feng Ming Ho, Chia Chi Chuang, Bo Lin Chen, Chun Fa Huang, Ya Wen Chen, Ruei Ming Chen, Shing Hwa Liu

研究成果: 雜誌貢獻文章同行評審

3 引文 斯高帕斯(Scopus)

摘要

The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

原文英語
頁(從 - 到)3258-3264
頁數7
期刊Acta Biomaterialia
5
發行號8
DOIs
出版狀態已發佈 - 十月 2009

ASJC Scopus subject areas

  • 生物技術
  • 生物材料
  • 生物化學
  • 生物醫學工程
  • 分子生物學

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