Tissue engineering-based cartilage repair with mesenchymal stem cells in a porcine model

Chih Hung Chang, Tzong Fu Kuo, Feng Huei Lin, Jyh Horng Wang, Yuan Ming Hsu, Huei Ting Huang, Shiao Tung Loo, Hsu Wei Fang, Hwa Chang Liu, Wen Chih Wang

研究成果: 雜誌貢獻文章

43 引文 斯高帕斯(Scopus)

摘要

This in vivo pilot study explored the use of mesenchymal stem cell (MSC) containing tissue engineering constructs in repair of osteochondral defects. Osteochondral defects were created in the medial condyles of both knees of 16 miniature pigs. One joint received a cell/collagen tissue engineering construct with or without pretreatment with transforming growth factor β (TGF-β) and the other joint from the same pig received no treatment or the gel scaffold only. Six months after surgery, in knees with no treatment, all defects showed contracted craters; in those treated with the gel scaffold alone, six showed a smooth gross surface, one a hypertrophic surface, and one a contracted crater; in those with undifferentiated MSCs, five defects had smooth, fully repaired surfaces or partially repaired surfaces, and one defect poor repair; in those with TGF-β-induced differentiated MSCs, seven defects had smooth, fully repaired surfaces or partially repaired surfaces, and three defects showed poor repair. In Pineda score grading, the group with undifferentiated MSC, but not the group with TGF-β-induced differentiated MSCs, had significantly lower subchondral, cell morphology, and total scores than the groups with no or gel-only treatment. The compressive stiffness was larger in cartilage without surgical treatment than the treated area within each group. In conclusion, this preliminary pilot study suggests that using undifferentiated MSCs might be a better approach than using TGF-β-induced differentiated MSCs for in vivo tissue engineered treatment of osteochondral defects.
原文英語
頁(從 - 到)1874-1880
頁數7
期刊Journal of Orthopaedic Research
29
發行號12
DOIs
出版狀態已發佈 - 十二月 1 2011
對外發佈Yes

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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    Chang, C. H., Kuo, T. F., Lin, F. H., Wang, J. H., Hsu, Y. M., Huang, H. T., Loo, S. T., Fang, H. W., Liu, H. C., & Wang, W. C. (2011). Tissue engineering-based cartilage repair with mesenchymal stem cells in a porcine model. Journal of Orthopaedic Research, 29(12), 1874-1880. https://doi.org/10.1002/jor.21461