TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum

Yu Jui Fan, Horn Jiunn Sheen, Zheng Yu Chen, Yi Hsing Liu, Jing Fa Tsai, Kuang Chong Wu

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highly sensitive for analyzing nanobead movements in the near-wall region. The characteristics of evanescent wave penetration of various liquid samples, which have different refractive indices, are investigated for nanobead illumination. The Brownian velocities of the nanobeads in various concentrations of CRPs within glycerol solution and serum are measured in real time. The detectable CRP concentration range of 1–6 mg/ml in human serum is found, and the detecting process can be finished in 10 min. From the results, the dissociation constant of CRP and anti-CRP in various viscosity solutions can be determined through the developed kinetic analysis.
原文英語
頁(從 - 到)85-94
頁數10
期刊Microfluidics and Nanofluidics
19
發行號1
DOIs
出版狀態已發佈 - 七月 15 2015
對外發佈Yes

指紋

serums
C-Reactive Protein
Fluorescence
proteins
Proteins
fluorescence
evanescent waves
glycerols
antibodies
Glycerol
Antibodies
Velocity measurement
Refractive index
penetration
Lighting
illumination
dissociation
Viscosity
refractivity
viscosity

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

引用此文

TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum. / Fan, Yu Jui; Sheen, Horn Jiunn; Chen, Zheng Yu; Liu, Yi Hsing; Tsai, Jing Fa; Wu, Kuang Chong.

於: Microfluidics and Nanofluidics, 卷 19, 編號 1, 15.07.2015, p. 85-94.

研究成果: 雜誌貢獻文章

Fan, Yu Jui ; Sheen, Horn Jiunn ; Chen, Zheng Yu ; Liu, Yi Hsing ; Tsai, Jing Fa ; Wu, Kuang Chong. / TIRF-enhanced nanobeads’ Brownian diffusion measurements for detecting CRP in human serum. 於: Microfluidics and Nanofluidics. 2015 ; 卷 19, 編號 1. 頁 85-94.
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abstract = "This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highly sensitive for analyzing nanobead movements in the near-wall region. The characteristics of evanescent wave penetration of various liquid samples, which have different refractive indices, are investigated for nanobead illumination. The Brownian velocities of the nanobeads in various concentrations of CRPs within glycerol solution and serum are measured in real time. The detectable CRP concentration range of 1–6 mg/ml in human serum is found, and the detecting process can be finished in 10 min. From the results, the dissociation constant of CRP and anti-CRP in various viscosity solutions can be determined through the developed kinetic analysis.",
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AU - Sheen, Horn Jiunn

AU - Chen, Zheng Yu

AU - Liu, Yi Hsing

AU - Tsai, Jing Fa

AU - Wu, Kuang Chong

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N2 - This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highly sensitive for analyzing nanobead movements in the near-wall region. The characteristics of evanescent wave penetration of various liquid samples, which have different refractive indices, are investigated for nanobead illumination. The Brownian velocities of the nanobeads in various concentrations of CRPs within glycerol solution and serum are measured in real time. The detectable CRP concentration range of 1–6 mg/ml in human serum is found, and the detecting process can be finished in 10 min. From the results, the dissociation constant of CRP and anti-CRP in various viscosity solutions can be determined through the developed kinetic analysis.

AB - This paper reports the in situ detection of C-reactive protein (CRP) and antibody-coated nanobead interaction by measuring the variation of the Brownian diffusion of the nanobeads. The measurements are carried out by using total internal reflective fluorescence-enhanced (TIRF-enhanced) micro-particle-tracking velocimetry (micro-PTV), which is highly sensitive for analyzing nanobead movements in the near-wall region. The characteristics of evanescent wave penetration of various liquid samples, which have different refractive indices, are investigated for nanobead illumination. The Brownian velocities of the nanobeads in various concentrations of CRPs within glycerol solution and serum are measured in real time. The detectable CRP concentration range of 1–6 mg/ml in human serum is found, and the detecting process can be finished in 10 min. From the results, the dissociation constant of CRP and anti-CRP in various viscosity solutions can be determined through the developed kinetic analysis.

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KW - TIRF

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