Tight regulation of a timed nuclear import wave of EKLF by PKCθ and FOE during Pro-E to Baso-E transition

Yu Chiau Shyu, Tung Liang Lee, Xin Chen, Pang Hung Hsu, Shau Ching Wen, Yi Wei Liaw, Chi Huan Lu, Po Yen Hsu, Mu Jie Lu, JauLang Hwang, Ming Daw Tsai, Ming Jing Hwang, Jim Ray Chen, Che Kun James Shen

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

Erythropoiesis is a highly regulated process during which BFU-E are differentiated into RBCs through CFU-E, Pro-E, PolyCh-E, OrthoCh-E, and reticulocyte stages. Uniquely, most erythroid-specific genes are activated during the Pro-E to Baso-E transition. We show that a wave of nuclear import of the erythroid-specific transcription factor EKLF occurs during the Pro-E to Baso-E transition. We further demonstrate that this wave results from a series of finely tuned events, including timed activation of PKCθ, phosphorylation of EKLF at S68 by P-PKCθ(S676), and sumoylation of EKLF at K74. The latter EKLF modifications modulate its interactions with a cytoplasmic ankyrin-repeat-protein FOE and importinβ1, respectively. The role of FOE in the control of EKLF nuclear import is further supported by analysis of the subcellular distribution patterns of EKLF in FOE-knockout mice. This study reveals the regulatory mechanisms of the nuclear import of EKLF, which may also be utilized in the nuclear import of other factors.

原文英語
頁(從 - 到)409-422
頁數14
期刊Developmental Cell
28
發行號4
DOIs
出版狀態已發佈 - 2月 24 2014
對外發佈

ASJC Scopus subject areas

  • 發展生物學

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