Thyroid hormone receptor-mediated regulation of the methionine adenosyltransferase 1 gene is associated with cell invasion in hepatoma cell lines

Sheng Ming Wu, Ya Hui Huang, Hsin Yi Lu, Ling Fang Chien, Chau Ting Yeh, Ming Chieh Tsai, Chen Hsin Liao, Wei Jan Chen, Chia Jung Liao, Wan Li Cheng, Kwang Huei Lin

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15 引文 斯高帕斯(Scopus)

摘要

The thyroid hormone T3 regulates differentiation, growth, and development. We demonstrated that methionine adenosyltransferase 1A (MAT1A) was positively regulated by T3 identified by cDNA microarray previously. The expression of the MAT1A was upregulated by T3 in hepatoma cell lines overexpressing thyroid hormone receptors (TRs). Additionally, these findings indicate that MAT1A may be regulated by CCAAT/enhancer binding protein (C/EBP). The critical role of the C/EBP binding sites was confirmed by the reporter or chromatin immuno-precipitation (ChIP) assay. In addition, C/EBP was upregulated in hepatoma cells after T3 treatment and ectopic expression of MAT1A inhibited cell migration and invasion in J7 hepatoma cells. Conversely, knockdown of MAT1A expression increased cell migration. Together, these findings suggest that the expression of the MAT1A gene is mediated by C/EBP and is indirectly upregulated by T3. Finally, TR was downregulated in a small subset of hepatocellular carcinoma cells concomitantly reduced the expression of C/EBPα and MAT1A.
原文英語
頁(從 - 到)1831-1843
頁數13
期刊Cellular and Molecular Life Sciences
67
發行號11
DOIs
出版狀態已發佈 - 6月 2010
對外發佈

ASJC Scopus subject areas

  • 細胞生物學
  • 分子生物學
  • 分子醫學
  • 藥理
  • 細胞與分子神經科學
  • 醫藥 (全部)

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