TY - JOUR
T1 - Thyroid hormone potentiates the antiviral action of interferon-γ in cultured human cells
AU - Lin, Hung Yun
AU - Thacore, Harshad R.
AU - Davis, Paul J.
AU - Davis, Faith B.
PY - 1994
Y1 - 1994
N2 - Human interferon-γ (hIFN γ) exhibits a number of biological effects, including antiviral activity in homologous cells. The antiviral activity of recombinant (r) hIFN γ, estimated by inhibition of vesicular stomatitis virus yield, was potentiated up to 120-fold in human fibroblast (BG-9) cells exposed to free L-T4 (0.5 × 10-10 mol/L). Thyroid hormone alone did not induce the antiviral state in BG-9 cells. L-T3 also potentiated the antiviral action of rhIFN γ, but D-T4 and D-T3 were ineffective. The antiviral effect of hIFN α in BG-9 cells was not influenced by thyroid hormone. Exposure of rhIFN γ-treated BG-9 cells to L-T4 for only 3 h was sufficient to potentiate hIFN γ-mediated antiviral activity. Similar potentiation by L-T4 of the antiviral effect of rhIFN γ in HeLa cell cultures was also observed. Although the mechanism of potentiation of rhIFN γ action by thyroid hormone is incompletely understood, the absence of antiviral activity of thyroid hormone alone indicates that the iodothyronine effect does not depend upon hormonal action on genes able to be stimulated by IFN γ.
AB - Human interferon-γ (hIFN γ) exhibits a number of biological effects, including antiviral activity in homologous cells. The antiviral activity of recombinant (r) hIFN γ, estimated by inhibition of vesicular stomatitis virus yield, was potentiated up to 120-fold in human fibroblast (BG-9) cells exposed to free L-T4 (0.5 × 10-10 mol/L). Thyroid hormone alone did not induce the antiviral state in BG-9 cells. L-T3 also potentiated the antiviral action of rhIFN γ, but D-T4 and D-T3 were ineffective. The antiviral effect of hIFN α in BG-9 cells was not influenced by thyroid hormone. Exposure of rhIFN γ-treated BG-9 cells to L-T4 for only 3 h was sufficient to potentiate hIFN γ-mediated antiviral activity. Similar potentiation by L-T4 of the antiviral effect of rhIFN γ in HeLa cell cultures was also observed. Although the mechanism of potentiation of rhIFN γ action by thyroid hormone is incompletely understood, the absence of antiviral activity of thyroid hormone alone indicates that the iodothyronine effect does not depend upon hormonal action on genes able to be stimulated by IFN γ.
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U2 - 10.1210/jcem.79.1.8027254
DO - 10.1210/jcem.79.1.8027254
M3 - Article
AN - SCOPUS:84995825964
VL - 79
SP - 62
EP - 65
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 1
ER -