Thrombomodulin mediates the progression of epithelial ovarian cancer cells

Lu Min Chen, Weu Wang, Jen Chih Lee, Feng Hsiang Chiu, Chun Te Wu, Cheng Jeng Tai, Chien Kai Wang, Chen Jei Tai, Ming Te Huang, Yu Jia Chang

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

Thrombomodulin (TM), a natural anticoagulation factor, maintains circulation homeostasis in endothelial cells. TM has additional roles in modulating inflammation, thrombosis, and carcinogenesis. However, there is little information on the role of TM in the progression and metastasis of ovarian cancer. RNA silencing and cDNA expression vectors were used to manipulate target gene expression in ovarian cancer cells. Cell growth and migration were evaluated by an MTT assay, a wound-healing migration assay, a transwell migration assay, and a biosensor system. In this study, we found that TM silencing may enhance the growth rate of cells. The migratory ability of ovarian cancer cells was enhanced dramatically after TM silencing. TM overexpression in ovarian cells suppressed the proliferation and migration capability. Furthermore, we found that skov-3 cells treated with TM shRNA expressed high levels of fibronectin and vimentin and that the expression of these markers correlated positively with their migratory ability. Our results demonstrate that TM expression may regulate cell growth and migration in ovarian cancer cells. This finding suggests that TM may be a novel prognostic and therapeutic target for ovarian cancer.

原文英語
頁(從 - 到)3743-3751
頁數9
期刊Tumor Biology
34
發行號6
DOIs
出版狀態已發佈 - 十二月 2013

ASJC Scopus subject areas

  • Cancer Research

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