Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection

Y. J. Fan, Y. C. Wu, Y. Chen, Y. C. Kung, T. H. Wu, K. W. Huang, H. J. Sheen, P. Y. Chiou

研究成果: 雜誌貢獻文章

18 引文 (Scopus)

摘要

We report a 3D microfluidic device with 32 detection channels and 64 sheath flow channels and embedded microball lens array for high throughput multicolor fluorescence detection. A throughput of 358?400 cells/s has been accomplished. This device is realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. High refractive index micro ball lenses (n?=?2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets.
原文繁體中文
期刊Biomicrofluidics
7
發行號4
DOIs
出版狀態已發佈 - 七月 1 2013
對外發佈Yes

引用此文

Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection. / Fan, Y. J.; Wu, Y. C.; Chen, Y.; Kung, Y. C.; Wu, T. H.; Huang, K. W.; Sheen, H. J.; Chiou, P. Y.

於: Biomicrofluidics, 卷 7, 編號 4, 01.07.2013.

研究成果: 雜誌貢獻文章

Fan, Y. J. ; Wu, Y. C. ; Chen, Y. ; Kung, Y. C. ; Wu, T. H. ; Huang, K. W. ; Sheen, H. J. ; Chiou, P. Y. / Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection. 於: Biomicrofluidics. 2013 ; 卷 7, 編號 4.
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