The zebrafish erythropoietin: Functional identification and biochemical characterization

Cheng Ying Chu, Chia Hsiung Cheng, Gen D. Chen, Yi Chung Chen, Chin Chun Hung, Kai Y. Huang, Chang J. Huang

研究成果: 雜誌貢獻文章同行評審

43 引文 斯高帕斯(Scopus)

摘要

In the present study, the zebrafish epo cDNA was cloned. The encoded protein displays 90%, 55% and 32% identity to the Epo from carp, fugu and human, respectively. Through RT-PCR, the expression of zepo mRNA was mainly in the heart and liver. In the COS-1 cell transfection experiments, the recombinant zEpo-HA protein was efficiently secreted into the culture medium as a glycoprotein and the carbohydrate moiety can be cleaved by the treatment of peptide-N-glycosidase F (PNGase F). Using the morpholino approach, we showed that zepo morphants displayed severe anemia leading to high mortality during development. Such an effect can be significantly rescued by zepo RNA. Furthermore, in the absence of functional zEpo, the expression of specific markers for adult globin genes, such as αA1- and βA1-globin, but not the embryonic βe1-globin, was affected.

原文英語
頁(從 - 到)4265-4271
頁數7
期刊FEBS Letters
581
發行號22
DOIs
出版狀態已發佈 - 九月 4 2007

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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