Background: Botulinum toxin type A (BTA) has been used on facial expressive muscles to reduce wrinkles. We assumed that intradermal injection with BTA on the middle and lower face can produce face-lifting and wrinkles-soothing effect by promoting collagen synthesis. Methods: Nine volunteers were included to undergo intradermal injections of a total dose of 20-25.U BTA into one-half of the face, and normal saline into the other half as control. Clinical photographs were taken every 4 weeks for 16 weeks, and evaluated by a doctor blinded to the assignments. Patients graded the results of wrinkles elimination on each follow-up. Skin biopsies were taken before the treatment and 8 weeks after the injection. The specimens were assessed by a pathologist using hematoxylin and eosin stain, elastin stain, Masson trichrome stain and immunohistochemical stain with type I procollagen. Results: By photographic documentation, there was no significant face-lifting effect. However, there was statistical significance in wrinkles reduction on the BTA sides compared to pre-treatment. Subjectively, six subjects noticed better wrinkles soothing effect on the BTA sides. This effect was noted as early as 4 weeks after injection, and lasted for a minimum 8 weeks. The histologic examination revealed slight neocollagen synthesis by Masson trichrome stain on both sides. Conclusions: Based on this pilot study, the face-lifting effect of intradermal injection with BTA was not conclusive. Interestingly, BTA showed moderate but significant wrinkles-soothing effect without obvious side effects on the lower face.
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Chang, S. P., Tsai, H. H., Chen, W. Y., Lee, W. R., Chen, P. L., & Tsai, T. H. (2008). The wrinkles soothing effect on the middle and lower face by intradermal injection of botulinum toxin type A. International Journal of Dermatology, 47(12), 1287-1294. https://doi.org/10.1111/j.1365-4632.2008.03895.x