The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture

Ming T. Lin, Wen Chi Su, Mei Ling Cheng, Kwang Shien Cheng, Wen Chang Chang, Lih Yuh C Wing, Chauyin J. Jen, Hua Lin Wu

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

The effects of fractionated oxidized low density lipoproteins (oxidized LDL) on the growth of vascular smooth muscle cells (VSMC) and their relationship to the formation of lysophosphatidylcholine (lyso-PC) as well as the activation of protein kinase C (PKC) were studied. VSMC were isolated from porcine aorta by explant culture. LDL was isolated from porcine blood by sequential ultracentrifugation and oxidized LDL was obtained by incubating LDL with 5 μMCuSO4 at 37 °C for various lengths of time. Our results showed that LDL oxidized for 12 h and eluted from fast protein liquid chromatography at 43 min inhibited the growth of VSMC, and that LDL oxidized for longer than 48 h and eluted at 48 min stimulated the growth of VSMC. The formation of lyso-PC in the oxidized LDL correlated well with its stimulatory effect, suggesting that lyso-PC is responsible for the mitogenic effect of oxidized LDL. This stimulatory effect of oxidized LDL was inhibited by staurosporine, a PKC inhibitor. Treatment with oxidized LDL increased the activity of membrane PKC, but it decreased that of cytosolic PKC, suggesting the translocation of PKC from cytosol to the membrane in the presence of oxidized LDL. These results suggested that the oxidized LDL-stimulated VSMC growth was mediated by the formation of lyso-PC and the activation of PKC.

原文英語
頁(從 - 到)260-268
頁數9
期刊Journal of Biomedical Science
6
發行號4
出版狀態已發佈 - 七月 1999
對外發佈Yes

指紋

Cell culture
Smooth Muscle Myocytes
Muscle
Cell Culture Techniques
Cells
Growth
Protein Kinase C
Vascular Smooth Muscle
Lysophosphatidylcholines
oxidized low density lipoprotein
Swine
Chemical activation
Staurosporine
Protein C Inhibitor
Ultracentrifugation
Liquid chromatography
Cell growth
Protein Kinase Inhibitors
Liquid Chromatography
Cytosol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

引用此文

Lin, M. T., Su, W. C., Cheng, M. L., Cheng, K. S., Chang, W. C., Wing, L. Y. C., ... Wu, H. L. (1999). The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture. Journal of Biomedical Science, 6(4), 260-268.

The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture. / Lin, Ming T.; Su, Wen Chi; Cheng, Mei Ling; Cheng, Kwang Shien; Chang, Wen Chang; Wing, Lih Yuh C; Jen, Chauyin J.; Wu, Hua Lin.

於: Journal of Biomedical Science, 卷 6, 編號 4, 07.1999, p. 260-268.

研究成果: 雜誌貢獻文章

Lin, MT, Su, WC, Cheng, ML, Cheng, KS, Chang, WC, Wing, LYC, Jen, CJ & Wu, HL 1999, 'The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture', Journal of Biomedical Science, 卷 6, 編號 4, 頁 260-268.
Lin, Ming T. ; Su, Wen Chi ; Cheng, Mei Ling ; Cheng, Kwang Shien ; Chang, Wen Chang ; Wing, Lih Yuh C ; Jen, Chauyin J. ; Wu, Hua Lin. / The various effects of fractionated oxidized low density lipoproteins on the growth of smooth muscle cells in culture. 於: Journal of Biomedical Science. 1999 ; 卷 6, 編號 4. 頁 260-268.
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abstract = "The effects of fractionated oxidized low density lipoproteins (oxidized LDL) on the growth of vascular smooth muscle cells (VSMC) and their relationship to the formation of lysophosphatidylcholine (lyso-PC) as well as the activation of protein kinase C (PKC) were studied. VSMC were isolated from porcine aorta by explant culture. LDL was isolated from porcine blood by sequential ultracentrifugation and oxidized LDL was obtained by incubating LDL with 5 μMCuSO4 at 37 °C for various lengths of time. Our results showed that LDL oxidized for 12 h and eluted from fast protein liquid chromatography at 43 min inhibited the growth of VSMC, and that LDL oxidized for longer than 48 h and eluted at 48 min stimulated the growth of VSMC. The formation of lyso-PC in the oxidized LDL correlated well with its stimulatory effect, suggesting that lyso-PC is responsible for the mitogenic effect of oxidized LDL. This stimulatory effect of oxidized LDL was inhibited by staurosporine, a PKC inhibitor. Treatment with oxidized LDL increased the activity of membrane PKC, but it decreased that of cytosolic PKC, suggesting the translocation of PKC from cytosol to the membrane in the presence of oxidized LDL. These results suggested that the oxidized LDL-stimulated VSMC growth was mediated by the formation of lyso-PC and the activation of PKC.",
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AU - Cheng, Kwang Shien

AU - Chang, Wen Chang

AU - Wing, Lih Yuh C

AU - Jen, Chauyin J.

AU - Wu, Hua Lin

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