The use of chitosan to enhance photodynamic inactivation against Candida albicans and its drug-resistant clinical isolates

Hsiung-Fei Chien, Chueh Pin Chen, Yee Chun Chen, Po Han Chang, Tsuimin Tsai, Chin Tin Chen

研究成果: 雜誌貢獻文章同行評審

34 引文 斯高帕斯(Scopus)

摘要

Drug-resistant Candida infection is a major health concern among immunocompromised patients. Antimicrobial photodynamic inactivation (PDI) was introduced as an alternative treatment for local infections. Although Candida (C.) has demonstrated susceptibility to PDI, high doses of photosensitizer (PS) and light energy are required, which may be harmful to eukaryotic human cells. This study explores the capacity of chitosan, a polycationic biopolymer, to increase the efficacy of PDI against C. albicans, as well as fluconazole-resistant clinical isolates in planktonic or biofilm states. Chitosan was shown to effectively augment the effect of PDI mediated by toluidine blue O (TBO) against C. albicans that were incubated with chitosan for 30 min following PDI. Chitosan at concentrations as low as 0.25% eradicated C. albicans; however, without PDI treatment, chitosan alone did not demonstrate significant antimicrobial activity within the 30 min of incubation. These results suggest that chitosan only augmented the fungicidal effect after the cells had been damaged by PDI. Increasing the dosage of chitosan or prolonging the incubation time allowed a reduction in the PDI condition required to completely eradicate C. albicans. These results clearly indicate that combining chitosan with PDI is a promising antimicrobial approach to treat infectious diseases.
原文英語
頁(從 - 到)7445-7456
頁數12
期刊International Journal of Molecular Sciences
14
發行號4
DOIs
出版狀態已發佈 - 四月 2013

ASJC Scopus subject areas

  • 電腦科學應用
  • 分子生物學
  • 催化
  • 無機化學
  • 光譜
  • 有機化學
  • 物理與理論化學
  • 醫藥 (全部)

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