Hollow, poly(L-lactic acid) microtube array membranes (MTAM) were used in preparing membranes that contained immobilized yeast cells. To evaluate the performance of the developed system for continuous and fed-batch fermentation, a gas chromatography/milli-whistle device was used to on-line monitor the production of ethanol. The milli-whistle was connected to the outlet of a GC capillary, and when the fermentation gases and the GC carrier gas passed through it, a sound with a fundamental frequency was produced. The online data obtained for frequency-change vs. retention time can be recorded after a fast Fourier transform. In typical bioethanol fermentation, the yeast cells cannot be recycled, whereas the artificial yeast-MTAMs can be. The hollow-MTAM containing immobilized yeast cells significantly enhanced to bioethanol productivity, and represent a novel, promising technology for bioethanol fermentation. Our data indicate that the gas chromatography/milli-whistle device, which is economical and stable, is a very useful detector for long-term monitoring.
|頁（從 - 到）||625-630|
|出版狀態||已發佈 - 2017|
ASJC Scopus subject areas
- Analytical Chemistry
Wu, M. J., Ye, G. F., Wang, C. H., Lin, H. T. V., Chen, C. C., & Lin, C. H. (2017). The use of a gas chromatography/milli-whistle technique for the on-line monitoring of ethanol production using microtube array membrane immobilized yeast cells. Analytical Sciences, 33(5), 625-630.