The ultrasensitive silver "protein" stain also detects nanograms of nucleic acids

Laura L. Somerville, Kuan Wang

研究成果: 雜誌貢獻文章同行評審

70 引文 斯高帕斯(Scopus)

摘要

The ultrasensitive silver staining procedure developed for proteins also stains nanogram quantities of RNA and DNA in polyacrylamide gels. A gradient polyacrylamide gel system is described which separates proteins from 104 to 106 Mr, RNA from 5S to 23S and DNA from 0.4 to 21 Kb. The sensitivity of nucleic acid silver staining in this gel system considerably exceeds that of commonly used DNA and RNA dye-binding stains.

原文英語
頁(從 - 到)53-58
頁數6
期刊Biochemical and Biophysical Research Communications
102
發行號1
DOIs
出版狀態已發佈 - 九月 16 1981
對外發佈Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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