The structural and functional contribution of N-terminal region and His-47 on Taiwan cobra phospholipase A2

Pei Hsiu Kao, Ku Chung Chen, Shinne Ren Lin, Long Sen Chang

研究成果: 雜誌貢獻文章同行評審

14 引文 斯高帕斯(Scopus)

摘要

Modification of His-47 and removal of the N-terminal octapeptide caused a different effect on the structure of Naja naja atra (Taiwan cobra) phospholipase A2 (PLA2). Unlike native enzyme, Ca2+ induced an alteration in the structural flexibility of His-modified PLA2. Moreover, the spatial positions of Trp residues in His-modified PLA2 were not properly rearranged toward lipid-water interface in the presence of Ca2+. CD spectra and fluorescence measurement showed that the dynamic properties of Trp residues and the gross conformation of N-terminally truncated PLA2 were totally different from native enzyme. Although a precipitous drop in the enzymatic activity was observed with modified PLA2. His-modified PLA2 and N-terminally truncated PLA2 retained cytotoxicity on inducing necrotic death of human neuroblastoma. SK-N-SH cells. Our data suggest that structural perturbations elicited by the chemical modification cause a dissociation of enzymatic activity and cytotoxicity of PLA2.

原文英語
頁(從 - 到)342-348
頁數7
期刊Journal of Peptide Science
14
發行號3
DOIs
出版狀態已發佈 - 三月 2008
對外發佈Yes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

指紋 深入研究「The structural and functional contribution of N-terminal region and His-47 on Taiwan cobra phospholipase A2」主題。共同形成了獨特的指紋。

引用此