The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

Lih Hwa S. Hwang, Bing Fung Chen, Huei Ming Lin, Lai Ling Soong, Yuh Shan Jou, Wen Chang Chang, Shih Tung Liu

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.
原文英語
頁(從 - 到)335-340
頁數6
期刊Biotechnology Letters
12
發行號5
DOIs
出版狀態已發佈 - 五月 1 1990
對外發佈Yes

指紋

Genetic Terminator Regions
Hormones
Escherichia coli
Growth Hormone
Swine
Initiator Codon
Nucleotides
Plasmids

ASJC Scopus subject areas

  • Biotechnology

引用此文

The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli. / Hwang, Lih Hwa S.; Chen, Bing Fung; Lin, Huei Ming; Soong, Lai Ling; Jou, Yuh Shan; Chang, Wen Chang; Liu, Shih Tung.

於: Biotechnology Letters, 卷 12, 編號 5, 01.05.1990, p. 335-340.

研究成果: 雜誌貢獻文章

Hwang, Lih Hwa S. ; Chen, Bing Fung ; Lin, Huei Ming ; Soong, Lai Ling ; Jou, Yuh Shan ; Chang, Wen Chang ; Liu, Shih Tung. / The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli. 於: Biotechnology Letters. 1990 ; 卷 12, 編號 5. 頁 335-340.
@article{3dc1ea16a921444f987699f43710af6e,
title = "The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli",
abstract = "Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.",
author = "Hwang, {Lih Hwa S.} and Chen, {Bing Fung} and Lin, {Huei Ming} and Soong, {Lai Ling} and Jou, {Yuh Shan} and Chang, {Wen Chang} and Liu, {Shih Tung}",
year = "1990",
month = "5",
day = "1",
doi = "10.1007/BF01024427",
language = "English",
volume = "12",
pages = "335--340",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "5",

}

TY - JOUR

T1 - The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone in Escherichia coli

AU - Hwang, Lih Hwa S.

AU - Chen, Bing Fung

AU - Lin, Huei Ming

AU - Soong, Lai Ling

AU - Jou, Yuh Shan

AU - Chang, Wen Chang

AU - Liu, Shih Tung

PY - 1990/5/1

Y1 - 1990/5/1

N2 - Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.

AB - Two types of expression vectors containing the Ptacor PLpromoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) in Escherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression of pGH in the Ptac-driven vectors, but reduced the synthesis of pGH in the PL-vectors. A leaky repression was observed in the JM103 cells containing the Ptac-plasmids.

UR - http://www.scopus.com/inward/record.url?scp=0025168953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025168953&partnerID=8YFLogxK

U2 - 10.1007/BF01024427

DO - 10.1007/BF01024427

M3 - Article

AN - SCOPUS:0025168953

VL - 12

SP - 335

EP - 340

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 5

ER -