The STAT3-miRNA-92-Wnt signaling pathway regulates spheroid formation and malignant progression in ovarian cancer

Min Wei Chen, Shu Ting Yang, Ming Hsien Chien, Kuo Tai Hua, Chin Jui Wu, S. M. Hsiao, Hao Lin, Michael Hsiao, Jen Liang Su, Lin Hung Wei

研究成果: 雜誌貢獻文章

50 引文 斯高帕斯(Scopus)

摘要

Ovarian cancer spheroids constitute a metastatic niche for transcoelomic spread that also engenders drug resistance. Spheroid-forming cells express active STAT3 signaling and display stem cell-like properties that may contribute to ovarian tumor progression. In this study, we show that STAT3 is hyperactivated in ovarian cancer spheroids and that STAT3 disruption in this setting is sufficient to relieve chemoresistance. In an NSG murine model of human ovarian cancer, STAT3 signaling regulated spheroid formation and self-renewal properties, whereas STAT3 attenuation reduced tumorigenicity. Mechanistic investigations revealed that Wnt signaling was required for STAT3-mediated spheroid formation. Notably, the Wnt antagonist DKK1 was the most strikingly upregulated gene in response to STAT3 attenuation in ovarian cancer cells. STAT3 signaling maintained stemness and interconnected Wnt/β-catenin signaling via the miR-92a/DKK1-regulatory pathways. Targeting STAT3 in combination with paclitaxel synergistically reduced peritoneal seeding and prolonged survival in a murine model of intraperitoneal ovarian cancer. Overall, our findings define a STAT3-miR-92a-DKK1 pathway in the generation of cancer stem-like cells in ovarian tumors, with potential therapeutic applications in blocking their progression.

原文英語
頁(從 - 到)1955-1967
頁數13
期刊Cancer Research
77
發行號8
DOIs
出版狀態已發佈 - 四月 15 2017

    指紋

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

引用此

Chen, M. W., Yang, S. T., Chien, M. H., Hua, K. T., Wu, C. J., Hsiao, S. M., Lin, H., Hsiao, M., Su, J. L., & Wei, L. H. (2017). The STAT3-miRNA-92-Wnt signaling pathway regulates spheroid formation and malignant progression in ovarian cancer. Cancer Research, 77(8), 1955-1967. https://doi.org/10.1158/0008-5472.CAN-16-1115