The small subunit ribosomal RNA gene sequence of Pleistophora anguillarum and the use of PCR primers for diagnostic detection of the parasite

Hsu Wei Hung, Chu Fang Lo, Chieh Chih Tseng, Shao En Peng, Chih Ming Chou, Guang Hsiung Kou

研究成果: 雜誌貢獻文章同行評審

15 引文 斯高帕斯(Scopus)

摘要

Using the polymerase chain reaction (PCR) and two primers for conserved regions of the small subunit ribosomal RNA (SSU-rRNA) of Microsporidia, a DNA segment about 1,195 base pairs long was amplified from a DNA template prepared from purified spores of the microsporidian species Pleistophora anguillarum. These spores had been isolated from adult eels (Anguilla japonica) with 'Beko Disease.' A comparison of sequence data from other microsporidian species showed P. anguillarum SSU-rRNA to be most similar to Vavraia oncoperae. When juvenile eels were artificially infected with P. anguillarum, enzyme-linked immunosorbent assay could detect a positive infection only 12 days post-infection. However, when suitable PCR primers were used, a DNA fragment of about 0.8 kb was detected from these juvenile eels after only 3 days post-infection. No PCR product was obtained with templates prepared from clinically healthy control animals.
原文英語
頁(從 - 到)556-560
頁數5
期刊Journal of Eukaryotic Microbiology
45
發行號5
出版狀態已發佈 - 9月 1998
對外發佈

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 應用微生物與生物技術
  • 農業與生物科學(雜項)
  • 微生物學

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