The sequential changes of the virion polypeptides of CV HB1 virus (Birnaviridae) during serial undiluted passaging

Chu Fang Lo, Shau Chi Chi, Nai Yueh Tien, Pei Jung Yu, Chih Ming Chou, Shiu Nan Chen, Chung Hsiung Wang, Guang Hsiung Kou

研究成果: 雜誌貢獻文章同行評審

摘要

Clam virus (CV HB-1) isolated from hard clam Meretrix lusoria, typed as infectious pancreatic necrosis virus (IPNV) Ab serotype, was employed for the present study. The structural polypeptides of purified virus generated by serial undiluted and diluted passaging were analyzed using sodiumdodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results indicated that in VP2, the virus particles of serial undiluted passaging had viral polypeptides smaller than those of serial diluted passaging. Furthermore, a polypeptide with a molecular mass of 105 kDa was found in the serial undiluted virus preparation. Further analysis by monoclonal antibodies showed that VP105 might be a polyprotein produced by genomic RNA segment A. To investigate the sequential changes of viral polypeptides of the virus preparation at high multiplicity infection, the viral polypeptides at each undiluted passage were analyzed by SDS-PAGE followed by immunoblotting. The results indicated that VP105 was present by undiluted passage 7, while the smaller VP2 was present by undiluted passage 10. The amount of smaller VP2 and VP105 increased as the number of passages increased.
原文英語
頁(從 - 到)73-80
頁數8
期刊Aquaculture
132
發行號1-2
DOIs
出版狀態已發佈 - 四月 15 1995
對外發佈

ASJC Scopus subject areas

  • 海洋科學

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