The roles of RGD and grooved topography in the adhesion, morphology, and differentiation of C2C12 skeletal myoblasts

Peng Yuan Wang, Helmut Thissen, Wei Bor Tsai

研究成果: 雜誌貢獻文章同行評審

60 引文 斯高帕斯(Scopus)

摘要

Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900nm in width with 600nm ridge spans and 665nm in depth were conjugated with the cell adhesion peptide arginine-glycine-aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering.
原文英語
頁(從 - 到)2104-2115
頁數12
期刊Biotechnology and Bioengineering
109
發行號8
DOIs
出版狀態已發佈 - 8月 2012
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 應用微生物與生物技術

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