The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma

Kai Chun Li, Ying Hsuan Huang, Ching Yin Ho, Chia Yu Chu, Shih Ting Cha, Hung Huey Tsai, Jenq Yuh Ko, Cheng Chi Chang, Ching Ting Tan

研究成果: 雜誌貢獻文章

14 引文 斯高帕斯(Scopus)

摘要

Stromal cell-derived factor-1 (SDF-1) (CXCL12) has been observed to promote laryngeal and hypopharyngeal squamous cell carcinomas (LHSCCs) invasion through cooperation with its receptor CXCR4. Here, we further explore the angiogenesis mechanism induced by SDF-1/CXCR4 interaction in LHSCCs. Immunohistochemistry (IHC) reveals the significant correlation between CXCR4 and angiogenesis in tumors. After blocking the function of CXCR4 by specific inhibitor AMD3100 and neutralized antibody 12G5 or inhibiting the expression by siRNA, we were able to disrupt the HUVECs tube formation, demonstrating that SDF-1/CXCR4 indeed regulated the angiogenesis mechanism. The angiogenesis profiling from angiogenesis array and reverse transcription polymerase chain reaction indicates that IL-8 can be significantly triggered by SDF-1/CXCR4 interaction in LHSCCs. We also demonstrate that IL-8 secretion mechanism is regulated by Akt phosphorylation after SDF-1 stimulation. These results point out the importance of SDF-1/CXCR4 interaction in LHSCCs angiogenesis. The angiogenic factor IL-8 would be triggered by the cooperation of SDF-1 and CXCR4 through an Akt-dependent pathway. This provides a new targeting therapy utility, disrupting SDF-1/CXCR4 interaction combined with downstream-induced angiogenic factors in LHSCCs would be beneficial to improve clinical outcome.
原文英語
頁(從 - 到)507-515
頁數9
期刊Oral Oncology
48
發行號6
DOIs
出版狀態已發佈 - 六月 2012

    指紋

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research

引用此

Li, K. C., Huang, Y. H., Ho, C. Y., Chu, C. Y., Cha, S. T., Tsai, H. H., Ko, J. Y., Chang, C. C., & Tan, C. T. (2012). The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma. Oral Oncology, 48(6), 507-515. https://doi.org/10.1016/j.oraloncology.2012.01.006