The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma

Kai Chun Li, Ying Hsuan Huang, Ching Yin Ho, Chia Yu Chu, Shih Ting Cha, Hung Huey Tsai, Jenq Yuh Ko, Cheng Chi Chang, Ching Ting Tan

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Stromal cell-derived factor-1 (SDF-1) (CXCL12) has been observed to promote laryngeal and hypopharyngeal squamous cell carcinomas (LHSCCs) invasion through cooperation with its receptor CXCR4. Here, we further explore the angiogenesis mechanism induced by SDF-1/CXCR4 interaction in LHSCCs. Immunohistochemistry (IHC) reveals the significant correlation between CXCR4 and angiogenesis in tumors. After blocking the function of CXCR4 by specific inhibitor AMD3100 and neutralized antibody 12G5 or inhibiting the expression by siRNA, we were able to disrupt the HUVECs tube formation, demonstrating that SDF-1/CXCR4 indeed regulated the angiogenesis mechanism. The angiogenesis profiling from angiogenesis array and reverse transcription polymerase chain reaction indicates that IL-8 can be significantly triggered by SDF-1/CXCR4 interaction in LHSCCs. We also demonstrate that IL-8 secretion mechanism is regulated by Akt phosphorylation after SDF-1 stimulation. These results point out the importance of SDF-1/CXCR4 interaction in LHSCCs angiogenesis. The angiogenic factor IL-8 would be triggered by the cooperation of SDF-1 and CXCR4 through an Akt-dependent pathway. This provides a new targeting therapy utility, disrupting SDF-1/CXCR4 interaction combined with downstream-induced angiogenic factors in LHSCCs would be beneficial to improve clinical outcome.
原文英語
頁(從 - 到)507-515
頁數9
期刊Oral Oncology
48
發行號6
DOIs
出版狀態已發佈 - 六月 2012

指紋

Chemokine CXCL12
Interleukin-8
Squamous Cell Carcinoma
Angiogenesis Inducing Agents
CXCR4 Receptors
Small Interfering RNA
Reverse Transcription
Immunohistochemistry
Phosphorylation
Polymerase Chain Reaction
Antibodies

ASJC Scopus subject areas

  • Oncology
  • Oral Surgery
  • Cancer Research

引用此文

The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma. / Li, Kai Chun; Huang, Ying Hsuan; Ho, Ching Yin; Chu, Chia Yu; Cha, Shih Ting; Tsai, Hung Huey; Ko, Jenq Yuh; Chang, Cheng Chi; Tan, Ching Ting.

於: Oral Oncology, 卷 48, 編號 6, 06.2012, p. 507-515.

研究成果: 雜誌貢獻文章

Li, KC, Huang, YH, Ho, CY, Chu, CY, Cha, ST, Tsai, HH, Ko, JY, Chang, CC & Tan, CT 2012, 'The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma', Oral Oncology, 卷 48, 編號 6, 頁 507-515. https://doi.org/10.1016/j.oraloncology.2012.01.006
Li, Kai Chun ; Huang, Ying Hsuan ; Ho, Ching Yin ; Chu, Chia Yu ; Cha, Shih Ting ; Tsai, Hung Huey ; Ko, Jenq Yuh ; Chang, Cheng Chi ; Tan, Ching Ting. / The role of IL-8 in the SDF-1α/CXCR4-induced angiogenesis of laryngeal and hypopharyngeal squamous cell carcinoma. 於: Oral Oncology. 2012 ; 卷 48, 編號 6. 頁 507-515.
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abstract = "Stromal cell-derived factor-1 (SDF-1) (CXCL12) has been observed to promote laryngeal and hypopharyngeal squamous cell carcinomas (LHSCCs) invasion through cooperation with its receptor CXCR4. Here, we further explore the angiogenesis mechanism induced by SDF-1/CXCR4 interaction in LHSCCs. Immunohistochemistry (IHC) reveals the significant correlation between CXCR4 and angiogenesis in tumors. After blocking the function of CXCR4 by specific inhibitor AMD3100 and neutralized antibody 12G5 or inhibiting the expression by siRNA, we were able to disrupt the HUVECs tube formation, demonstrating that SDF-1/CXCR4 indeed regulated the angiogenesis mechanism. The angiogenesis profiling from angiogenesis array and reverse transcription polymerase chain reaction indicates that IL-8 can be significantly triggered by SDF-1/CXCR4 interaction in LHSCCs. We also demonstrate that IL-8 secretion mechanism is regulated by Akt phosphorylation after SDF-1 stimulation. These results point out the importance of SDF-1/CXCR4 interaction in LHSCCs angiogenesis. The angiogenic factor IL-8 would be triggered by the cooperation of SDF-1 and CXCR4 through an Akt-dependent pathway. This provides a new targeting therapy utility, disrupting SDF-1/CXCR4 interaction combined with downstream-induced angiogenic factors in LHSCCs would be beneficial to improve clinical outcome.",
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AU - Chu, Chia Yu

AU - Cha, Shih Ting

AU - Tsai, Hung Huey

AU - Ko, Jenq Yuh

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AU - Tan, Ching Ting

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AB - Stromal cell-derived factor-1 (SDF-1) (CXCL12) has been observed to promote laryngeal and hypopharyngeal squamous cell carcinomas (LHSCCs) invasion through cooperation with its receptor CXCR4. Here, we further explore the angiogenesis mechanism induced by SDF-1/CXCR4 interaction in LHSCCs. Immunohistochemistry (IHC) reveals the significant correlation between CXCR4 and angiogenesis in tumors. After blocking the function of CXCR4 by specific inhibitor AMD3100 and neutralized antibody 12G5 or inhibiting the expression by siRNA, we were able to disrupt the HUVECs tube formation, demonstrating that SDF-1/CXCR4 indeed regulated the angiogenesis mechanism. The angiogenesis profiling from angiogenesis array and reverse transcription polymerase chain reaction indicates that IL-8 can be significantly triggered by SDF-1/CXCR4 interaction in LHSCCs. We also demonstrate that IL-8 secretion mechanism is regulated by Akt phosphorylation after SDF-1 stimulation. These results point out the importance of SDF-1/CXCR4 interaction in LHSCCs angiogenesis. The angiogenic factor IL-8 would be triggered by the cooperation of SDF-1 and CXCR4 through an Akt-dependent pathway. This provides a new targeting therapy utility, disrupting SDF-1/CXCR4 interaction combined with downstream-induced angiogenic factors in LHSCCs would be beneficial to improve clinical outcome.

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