The protective effect of inflammatory monocytes during systemic C. Albicans infection is dependent on collaboration between C-type lectin-like receptors

Aiysha Thompson, Luke C. Davies, Chia Te Liao, Diogo M. da Fonseca, James S. Griffiths, Robert Andrews, Adam V. Jones, Mathew Clement, Gordon D. Brown, Ian R. Humphreys, Philip R. Taylor, Selinda J. Orr

研究成果: 雜誌貢獻文章同行評審

18 引文 斯高帕斯(Scopus)

摘要

Invasive candidiasis, mainly caused by Candida albicans, is a serious healthcare problem with high mortality rates, particularly in immunocompromised patients. Innate immune cells express pathogen recognition receptors (PRRs) including C-type lectin-like receptors (CLRs) that bind C. albicans to initiate an immune response. Multiple CLRs including Dectin-1, Dectin-2 and Mincle have been proposed individually to contribute to the immune response to C. albicans. However how these receptors collaborate to clear a fungal infection is unknown. Herein, we used novel multi-CLR knockout (KO) mice to decipher the individual, collaborative and collective roles of Dectin-1, Dectin-2 and Mincle during systemic C. albicans infection. These studies revealed an unappreciated and profound role for CLR cooperation in anti-fungal immunity. The protective effect of multiple CLRs was markedly greater than any single receptor, and was mediated through inflammatory monocytes via recognition and phagocytosis of C. albicans, and production of C. albicans-induced cytokines and chemokines. These CLRs were dispensable for mediating similar responses from neutrophils, likely due to lower expression of these CLRs on neutrophils compared to inflammatory monocytes. Concurrent deletion of Dectin-1 and Dectin-2, or all three CLRs, resulted in dramatically increased susceptibility to systemic C. albicans infection compared to mice lacking a single CLR. Multi-CLR KO mice were unable to control fungal growth due to an inadequate early inflammatory monocyte-mediated response. In response to excessive fungal growth, the multi-CLR KO mice mounted a hyper-inflammatory response, likely leading to multiple organ failure. Thus, these data reveal a critical role for CLR co-operation in the effective control of C. albicans and maintenance of organ function during infection.

原文英語
文章編號e1007850
期刊PLoS Pathogens
15
發行號6
DOIs
出版狀態已發佈 - 六月 2019
對外發佈

ASJC Scopus subject areas

  • 寄生物學
  • 微生物學
  • 免疫學
  • 分子生物學
  • 遺傳學
  • 病毒學

指紋

深入研究「The protective effect of inflammatory monocytes during systemic C. Albicans infection is dependent on collaboration between C-type lectin-like receptors」主題。共同形成了獨特的指紋。

引用此