The protective effect of human platelet lysate in models of neurodegenerative disease: Involvement of the Akt and MEK pathways

Flore Gouel, Bruce Do Van, Ming Li Chou, Aurélie Jonneaux, Caroline Moreau, Régis Bordet, Thierry Pierre Robert Burnouf, Jean Christophe Devedjian, David Devos

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20 引文 斯高帕斯(Scopus)

摘要

Neurodegenerative diseases have huge economic and societal impacts, and place an immense emotional burden on patients and caregivers. Given that platelets have an essential physiological role in wound healing and tissue repair, human platelet lysates (HPLs) are being developed as a novel, effective biotherapy for neurodegenerative diseases. HPLs constitute abundant, readily accessible sources of physiological mixtures of many growth factors (GFs), with demonstrable effects on neuron survival and thus the development, maintenance, function and plasticity of the vertebrate nervous system. Here, we found that HPLs had marked neuroprotective abilities in cell-based models of Parkinson's disease and amyotrophic lateral sclerosis (the LUHMES and NSC-34 cell lines, respectively). The HPLs protected against specific cell death pathways (apoptosis and ferroptosis) and specific oxidative stress inducers [1-methyl-4-phenylpyridinium (MPP+) and menadione], and always afforded more protection than commonly used recombinant GFs (rGFs). The mechanism of protection of HPLs involved specific signalling pathways: whereas the Akt pathway was activated by HPLs under all conditions, the MEK pathway appeared to be more specifically involved in protection against MPP+ toxicity in LUHMES and, in a lesser extent, in staurosporine toxicity in NSC-34. Our present results suggest that HPLs-based therapies could be used to prevent neuronal loss in neurodegenerative diseases while overcoming the limitations currently associated with use of rGFs.
原文英語
頁(從 - 到)3236-3240
頁數5
期刊Journal of Tissue Engineering and Regenerative Medicine
11
發行號11
DOIs
出版狀態已發佈 - 2017

ASJC Scopus subject areas

  • 醫藥(雜項)
  • 生物材料
  • 生物醫學工程

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