The propeller DNA conformation of poly(dA).poly(dT)

Joan Aymami, Miquel Coll, Christin A. Frederick, Andrew H.J. Wang, Alexander Rich

研究成果: 雜誌貢獻文章同行評審

62 引文 斯高帕斯(Scopus)

摘要

Physical properties of the DNA duplex, poly(dA).poly(dT) differ considerably from the alternating copolymer poly(dAT). A number of molecular models have been used to describe these structures obtained from fiber X-ray diffraction data. The recent solutions of single crystal DNA dodecamer structures with segments of oligo-A.oligo-T have revealed the presence of a high propeller twist in the AT regions which is stabilized by the formation of bifurcated (three-center) hydrogen bonds on the floor of the major groove, involving the N6 amino group of adenine hydrogen bonding to two 04 atoms of adjacent thymine residues on the opposite strand. Here we show that it is possible to incorporate the features of the single crystal analysis, specifically high propeller twist, bifurcated hydrogen bonds, and a narrow minor groove, as well as the close interstrand N signal between adenine HC2 and ribose HC1' of the opposite strand, into a model that is fully compatible with the diffraction data obtained from poly(dA) poly(dT).

原文英語
頁(從 - 到)3229-3245
頁數17
期刊Nucleic Acids Research
17
發行號8
DOIs
出版狀態已發佈 - 4月 25 1989
對外發佈

ASJC Scopus subject areas

  • 遺傳學

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