Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.
ASJC Scopus subject areas
- Materials Science(all)
Chen, W. H., Liu, H. Y., Tsai, C. Y., Wu, C. C., Wei, H. J., Liu, A., Lai, M-T., Huang, C. F., & Deng, W. P. (2015). The potential use of platelet-rich plasma to reconstruct the microtia chondrocyte in human auricular cartilage regeneration. Journal of Nanomaterials, 2015, . https://doi.org/10.1155/2015/250615